Transcriptomics

Dataset Information

0

ENL reads histone β-hydroxybutyrylation to modulate gene transcription (RNA-Seq)


ABSTRACT: Histone modifications are typically recognized by chromatin-binding protein modules (referred to as “readers”) to mediate fundamental processes such as transcription. Lysine β-hydroxybutyrylation (Kbhb) is a new type of histone mark that couples metabolism to gene expression. However, the readers that prefer histone Kbhb remain elusive. This knowledge gap must be filled in order to reveal the molecular mechanism of this epigenetic regulation. Herein, we developed a chemical proteomic approach, relying upon multivalent photoaffinity probes to capture binders of the mark and identified ENL as a novel target of H3K9bhb. Biochemical studies and CUT&Tag analysis further suggested that ENL favorably binds to H3K9bhb, and co-localizes with it on promoter regions to modulate gene expression. Notably, disrupting the interaction between H3K9bhb and ENL via structure-based mutation leads to the suppressed expression of the gene like MYC that drives cell proliferation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE266269 | GEO | 2024/05/06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-05-06 | GSE266268 | GEO
2016-04-06 | E-GEOD-69616 | biostudies-arrayexpress
2016-04-06 | E-GEOD-69615 | biostudies-arrayexpress
2016-04-06 | GSE69616 | GEO
2016-04-06 | GSE69615 | GEO
2021-11-23 | GSE122896 | GEO
2021-09-08 | GSE175731 | GEO
2024-07-04 | GSE179410 | GEO
2021-09-08 | GSE176359 | GEO
2023-03-14 | PXD034269 |