Transcriptomics

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Poly-A transcriptome changes in mouse embryonic fibroblasts (MEFs) after spontaneous immortalization


ABSTRACT: In our study, we characterized the changes in poly-A gene expression atributed to the process of spontaneous immortalization using a mouse embryonic fibroblasts (MEF) model. Primary MEFs were established, serially passaged and spontaneously immortalized using a modified 3T3 NIH protocol. We then performed gene expression profiling analysis using RNA-seq data of 3 different clones at two time points (primary and immortalized).

ORGANISM(S): Mus musculus

PROVIDER: GSE266445 | GEO | 2024/08/27

REPOSITORIES: GEO

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