Dataset Information

Transcriptome dynamics in the Arabidopsis male germline during pollen-pistil interactions

ABSTRACT: When pollen lands on a receptive stigma, it germinates and extends a tube inside the transmitting tissue of the pistil to deliver the sperm cells for double fertilization. The growth of the pollen tube triggers significant alterations in its gene expression. The extent to which these changes occur in the vegetative cell or extend to the sperm cells transported by the tube is unclear, but important to determine since sperm cells are believed to acquire a competency for fertilization during pollen-pistil interactions. To address these questions, we compared the transcriptomes of Arabidopsis thaliana sperm cells and vegetative nuclei isolated from mature pollen grains with those isolated from in vitro grown pollen tubes. Importantly, we also compared with transcriptomes of sperm cells obtained from pollen tubes grown under semi in vivo conditions where tubes passed through a pistil section. Our data shows that extensive transcriptomic changes occur in sperm cells during pollen tube growth, some of which are elicited only as sperms are carried through the pistil. Their analysis reveals a host of previously unidentified transcripts that may facilitate sperm maturation and gamete fusion. The vegetative cell undergoes even more extensive transcriptomic reprogramming during pollen tube growth, mainly through the upregulation of genes associated with pollen tube growth and vesicle-mediated transport. Interestingly, ATAC-seq data shows that the promoters of genes up-regulated in sperm during pollen tube growth are already accessible in sperm chromatin of mature pollen grains, suggesting pre-configured promoter accessibility. This study's expression data can be further explored here: https://bar.utoronto.ca/eFP-Seq_Browser/.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE266759 | GEO | 2025/03/16

REPOSITORIES: GEO

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Project description:Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step towards a detailed understanding of this important process, the objective of this study was to comprehensively analyze the transcriptome changes during pollen germination and pollen tube growth. Using Affymetrix Arabidopsis ATH1 Genome Arrays, this study is the first to show the changes in the transcriptome from desiccated mature pollen grains to hydrated pollen grains and then to pollen tubes of Arabidopsis thaliana. The number of expressed genes, either for total expressed genes or for specifically expressed genes, increased significantly from desiccated mature pollen to hydrated pollen and again to growing pollen tubes, which is consistent with the finding that pollen germination and tube growth was significantly inhibited in vitro by a transcriptional inhibitor. The results of GO analyses showed that expression of genes related to cell rescue, transcription, signal transduction and cellular transport were significantly changed, especially for up-regulation, during pollen germination and tube growth, respectively. In particular, genes of the CaM/CML, CHX and Hsp families showed the most significant changes during pollen germination and tube growth. These results demonstrate that the overall transcription of genes, both in the number of expressed genes and in the levels of transcription, was increased. Furthermore, the appearance of many novel transcripts during pollen germination as well as tube growth indicates that these newly expressed genes may function in this complex process. SUBMITTER_CITATION: Yi Wang, Wen-Zheng Zhang, Lian-Fen Song, Jun-Jie Zou, Zhen Su, and Wei-Hua Wu. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol. September 5, 2008; 10.1104/pp.108.126375 Experiment Overall Design: Three samples are analyzed in this experiment. They are desiccated mature pollen grains (MP), hydrated pollen grains (HP) and growing pollen tubes (PT) of Arabidopsis thaliana, respectively. Each sample has two biological replicates, so that there are 6 data sets of ATH1 array in this experiment.

Project description:As in animals, cell-cell communication plays pivotal role in male-female recognition during plant sexual reproduction. Prelaid peptides secreted from the female reproductive tissues guide pollen tubes towards ovules for fertilization. However, the elaborate mechanisms for this dialogue have remained elusive, particularly from the male perspective. We perform genome-wide quantitative liquid chromatography coupled tandem mass spectrometry of a pistil-stimulated pollen tube secretome and identify 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube-secretome is dominated by unconventional-type secreted proteins representing 57% of the total secretome. In support, we show that unconventional-type protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discover that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that Arabidopsis thaliana translationally controlled tumor protein-knockdown plants have pollen tubes that poorly navigate to the target ovule, and theÂ knocked down allele is poorly transmitted through the male. We show that regulators of the endoplasmic reticulum-trans-golgi network protein secretory pathway control secretion of pollen tube-secreted cysteine-rich proteins, including pollen tube attractants, and are essential for pollen tube growth and guidance, as well as ovule-targeting competence. This work, the first pollen tube secretome study, identifies novel genome-wide pollen tube-secreted proteins with potential function in pollen tube-ovule guidance for sexual reproduction. Functional analysis highlights a potential mechanism for pollen tube unconventional protein secretion and reveals likely regulators of pollen tube protein secretion. The association of pollen tube-secreted proteins with marker proteins shown to be secreted via exosomes in other species suggest secretion via exosomes as a possible mechanism for cell-cell communication between the pollen tube and female reproductive cells. For processed dataset with quantitative information, see Hafidh S, Potesil D, Fila J et al. Genome Bilogy 2016.