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Fluo-Cast-Bright: A Deep Learning Pipeline for the Non-Invasive Prediction of Chromatin Structure and Developmental Potential in Live Oocytes

ABSTRACT: In mammalian oocytes, large-scale chromatin organization regulates transcription, nuclear architecture, and maintenance of chromosome stability in preparation for meiosis onset. Pre-ovulatory oocytes with distinct chromatin configuration exhibit profound differences in metabolic and transcriptional profiles that ultimately determine meiotic competence and developmental potential. Here, we developed a deep learning pipeline for the non-invasive prediction of chromatin structure and developmental potential in live mouse oocytes. Our Fluorescence prediction and Classification on Bright-field (Fluo-Cast-Bright) pipeline achieved 91.3% accuracy in the classification of chromatin state in fixed oocytes and 85.7% accuracy in live oocytes. Importantly, transcriptome analysis following non-invasive selection revealed that meiotically competent oocytes exhibit a higher expression of transcripts associated with RNA and protein nuclear export, maternal mRNA deadenylation, histone modifications, chromatin remodeling and signaling pathways regulating microtubule dynamics during the metaphase-I to metaphase-II transition. Our pipeline provides fast and non-invasive selection of meiotically competent oocytes for downstream research and clinical applications.

ORGANISM(S): Mus musculus

PROVIDER: GSE266845 | GEO | 2025/01/29

REPOSITORIES: GEO

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