Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA Sequencing Gene Expression of Endothelial differentiation in DMP1 treated DPSCs.

ABSTRACT: Regenerative vascular medicine has received considerable attention due to recent advancements suggesting that the conversion of somatic cells into endothelial cells offers a promising approach to address diseases related to abnormal vessel growth and endothelial cell dysfunction. The therapeutic potential of this method lies in the ability of DPSCs, upon exposure to DMP1, to differentiate into endothelial cells and support the formation of new blood vessels. Despite this progress, there is a need for a comprehensive understanding of the expression profile during endothelial differentiation. Gene ontology and genome functional enrichment analyses have provided insight into the differentiation of ECs from DMP1-treated DPSCs in conjunction with HUVEC-ECM, as compared to positive HUVECs. Our RNA sequence data underscores the striking similarity in gene expression patterns between the sorted DPSCs treated with DMP1 and the positive control HUVECs. These findings could contribute to a comprehensive understanding of the molecular mechanisms underlying the impact of DMP1 treatment on DPSCs, particularly in the context of guiding endothelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE266875 | GEO | 2024/05/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA Sequencing Gene Expression of Endothelial differentiation in DMP1 treated DPSCs.

Project description:RNA Sequencing Gene Expression of Endothelial differentiation in DMP1 treated DPSCs.

| PRJNA1108653 | ENA

Single-cell RNA-sequencing of intratibial LLC cancer model in DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice.

Project description:We injected LLC1 cells into the tibia of DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice to study the changes in the cellular composition of the cancer microenvironment after knockout of Mfn2 or Rhot1 in osteocytes.

2024-01-01 | GSE228708 | GEO

Role of FGF23 and DMP1 in osteoblasts

Project description:Primary bone marrow cells from murine long bones were cultured in osteoblastic medium for 21 days. RNAseq and differential expression analyzes were used to highlight the importance of FGF23 and DMP1 in osteoblastogenesis

2024-01-31 | GSE239825 | GEO

Expression data from Dmp1-GFP sorted osteocytes

Project description:Estrogens are well known steroid hormones necessary to maintain bone health. In addition, mechanical loading, which estrogen signaling may intersect with the Wnt/Î²-catenin pathway, is also essential for bone health. As osteocytes are known as the major mechanosensory cells embedded in mineralized bone matrix, osteocyte ERÎ± deletion mice (ERÎ±ÎOcy/ÎOcy) were generated by mating ERÎ± floxed mice with Dmp1-Cre mice to determine functions of ERÎ± in osteocytes. Trabecular bone mineral density of female, but not male ERÎ±ÎOcy/ÎOcy mice was significantly decreased. Bone formation parameters in ERÎ±ÎOcy/ÎOcy were significantly decreased while osteoclast parameters were unchanged. This suggests that ERÎ± in osteocytes exerts osteoprotective function by positively controlling bone formation. To identify potential targets of ERÎ±, gene array analysis of Dmp1-GFP osteocytes FACS sorted from ERÎ±ÎOcy/ÎOcy and control mice was performed. Expression of Mdk and Sostdc1, both known inhibitors of Wnt, were significantly increased without alteration of the mature osteocyte marker Sost or Î²-catenin. Hindlimb unloading exacerbated the trabecular bone loss, but surprisingly cortical bone was resistant. These studies show that ERÎ± in osteocytes has osteoprotective effects in trabecular bone through regulating expression of Wnt antagonists, but conversely plays a negative role in cortical bone loss due to unloading. Wild type and osteocyte-specific Estrogen Receptor alpha knock-out mice were generated. The number of both genotypes of mice was three. Calvarial osteocytes of both genotypes harboring Dmp1-GFP were extracted by sequential enzymatic digestion, followed by FACS Aria sorting and total RNAs were purified for Affymetix GeneChip microarray analysis without pooling.

2013-12-12 | E-GEOD-41997 | biostudies-arrayexpress

Expression data from Dmp1-Cre targeted and non-targeted Cxcl12-abundant reticular (CAR) cells in mouse bone marrow

Project description:In mouse bone marrow, mesenchymal stem cells (MSC) has the potential to form osteocytes, adipocytes and cartilage. In the process of osteogenesis, MSCs differenetiate into stromal cells, such as CAR cells. Osteoblast is responsible for the formation of osteocytes and osteoblasts may be differentiated from a subset of CAR cells. Dmp1-Cre targeted CAR cells are thought to enrich for a osteoblast progenitor population. We used microarrays to detail the gene expression profiles among Dmp1-Cre targeted and non-targeted CAR cells. Gene expression diffferences were compared to support the hypothesis that Dmp1-Cre targeted CAR cells may be enriched for osteoblast progenitors. Dmp1-Cre targeted and non-targeted CAR cells were FACS sorted from three mice. RNA were extracted from these sorted cells and processed for microarray using Affymetrix mogene 1.0 ST chip. Cells from one mouse represent one sample

2016-05-14 | E-GEOD-81399 | biostudies-arrayexpress

Expression data from Dmp1-Cre targeted and non-targeted Cxcl12-abundant reticular (CAR) cells in mouse bone marrow

2016-05-14 | GSE81399 | GEO

Role of FGF23 and DMP1 in osteoblasts

Project description:Role of FGF23 and DMP1 in osteoblasts

| PRJNA1001031 | ENA

Role of FGF23 and DMP1 in osteoblastogenesis

Project description:Role of FGF23 and DMP1 in osteoblastogenesis

| PRJNA1001027 | ENA

Role of FGF23 and DMP1 in osteoblastogenesis in cultured BMSCs at single cell level

Project description:Primary bone marrow cells from murine long bones were cultured in osteoblastic medium for 21 days. scRNAseq and differential expression analyzes were used to highlight the importance of FGF23 and DMP1 in osteoblastogenesis

2024-01-31 | GSE239828 | GEO

Expression data from Dmp1-GFP sorted osteocytes

Project description:Estrogens are well known steroid hormones necessary to maintain bone health. In addition, mechanical loading, which estrogen signaling may intersect with the Wnt/β-catenin pathway, is also essential for bone health. As osteocytes are known as the major mechanosensory cells embedded in mineralized bone matrix, osteocyte ERα deletion mice (ERαΔOcy/ΔOcy) were generated by mating ERα floxed mice with Dmp1-Cre mice to determine functions of ERα in osteocytes. Trabecular bone mineral density of female, but not male ERαΔOcy/ΔOcy mice was significantly decreased. Bone formation parameters in ERαΔOcy/ΔOcy were significantly decreased while osteoclast parameters were unchanged. This suggests that ERα in osteocytes exerts osteoprotective function by positively controlling bone formation. To identify potential targets of ERα, gene array analysis of Dmp1-GFP osteocytes FACS sorted from ERαΔOcy/ΔOcy and control mice was performed. Expression of Mdk and Sostdc1, both known inhibitors of Wnt, were significantly increased without alteration of the mature osteocyte marker Sost or β-catenin. Hindlimb unloading exacerbated the trabecular bone loss, but surprisingly cortical bone was resistant. These studies show that ERα in osteocytes has osteoprotective effects in trabecular bone through regulating expression of Wnt antagonists, but conversely plays a negative role in cortical bone loss due to unloading.

2013-12-12 | GSE41997 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data