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The effect of metal cations on the capture of Pol II-derived nascent transcripts in NET-seq experiments

ABSTRACT: The goal of this study was to test the effect of metal cations (CaCl2 and MnCl2) during Pol II NET-seq. These metals are readily used in the majority of studies that use NET-seq to analyze Pol II occupancy, but their effect on nascent transcript capture has not been analyzed. Our results suggest that the inclusion of these metals in Pol II NET-seq experiments does not cause a significant change in Pol II occupancy in the untreated control vs. metal-treated (CaCl2 or MnCl2) samples.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE267082 | GEO | 2024/05/13

REPOSITORIES: GEO

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