Transcriptomics

Dataset Information

0

Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells (mRNA)


ABSTRACT: Epigenetic modification of the mammalian genome by DNA methylation (5-methylcytosine) has a profound impact on chromatin structure, gene expression and maintenance of cellular identity. Recent demonstration that members of the Ten-eleven translocation (Tet) family proteins can convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) raised the possibility that Tet proteins are capable of establishing a distinct epigenetic state. We have recently demonstrated that Tet1 is specifically expressed in murine embryonic stem (ES) cells and is required for ES cell self-renewal and maintenance. Using chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq), here we show that Tet1 is preferentially bound to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Despite a general increase in levels of DNA methylation at Tet1 binding-sites, Tet1 depletion does not lead to down-regulation of all the Tet1 targets. Interestingly, while Tet1-mediated promoter hypomethylation is required for maintaining the expression of a group of transcriptionally active genes, it is also required for repression of Polycomb-targeted developmental regulators. Tet1 contributes to silencing of this group of genes by facilitating recruitment of PRC2 to CpG-rich gene promoters. Thus, our study not only establishes a role for Tet1 in modulating DNA methylation levels at CpG-rich promoters, but also reveals a dual function of Tet1 in promoting transcription of pluripotency factors as well as participating in the repression of Polycomb-targeted developmental regulators.

ORGANISM(S): Mus musculus

PROVIDER: GSE26830 | GEO | 2011/03/30

SECONDARY ACCESSION(S): PRJNA142017

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-03-30 | E-GEOD-26832 | biostudies-arrayexpress
2011-03-30 | E-GEOD-26830 | biostudies-arrayexpress
2011-03-30 | E-GEOD-26827 | biostudies-arrayexpress
2011-03-30 | GSE26832 | GEO
2011-03-30 | GSE26827 | GEO
2024-06-04 | GSE268886 | GEO
2024-06-04 | GSE268885 | GEO
2024-06-04 | GSE268884 | GEO
2011-04-21 | E-GEOD-28533 | biostudies-arrayexpress
2013-06-30 | E-GEOD-46402 | biostudies-arrayexpress