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Methylome comparisons between Red-Flesh and White-Flesh Mesocarps in Red-Flesh apple at 84 Days After Full Bloom (DAFB)

ABSTRACT: To functionally characterise flesh pigmentation pattern, we performed methylation analysis between RF and WF mesocarps. The transition step between fully red fruits and heterogeneously pigmented fruit were targeted during fruit development (84 Days After Full Bloom - DAFB).

ORGANISM(S): Malus domestica

PROVIDER: GSE269807 | GEO | 2024/06/18

REPOSITORIES: GEO

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Transcriptomic analysis between Red-Flesh and White-Flesh Mesocarps in Red-Flesh apple at 84 Days After Full Bloom (DAFB)

Project description:To functionally characterise flesh pigmentation pattern, we performed transcriptomic comparisons between RF and WF mesocarps. The transition step between fully red fruits and heterogeneously pigmented fruit were targeted during fruit development (84 Days After Full Bloom - DAFB).

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Methylome comparisons between Red-Flesh and White-Flesh Mesocarps in Red-Flesh apple at 84 Days After Full Bloom (DAFB)

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Transcriptomic analysis between Red-Flesh and White-Flesh Mesocarps in Red-Flesh apple at 84 Days After Full Bloom (DAFB)

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Microarray analysis of fruit flesh of European pear M-bM-^@M-^XLa FranceM-bM-^@M-^Y during fruit development

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2013-12-18 | E-GEOD-48393 | biostudies-arrayexpress

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Project description:Bud mutation (bud sport) is mutation occurred in one branch in a tree and the mutant branch shows different phenotype from normal branches. Because bud mutation occurs in a part of plant body, genomic backgrounds of mutant branch and normal branches are the same, except some mutations. Therefore bud mutants are ideal material to identify key genes for important traits of crops. We studied ‘Giant Laf’, a bud mutant setting large fruit, which generated spontaneously in European pear ‘La France’ tree. In ‘Giant Laf’, increase of cell size and DNA reduplication occurred specifically in fruit flesh. The DNA reduplication in ‘Giant Laf’ was observed at 1 week before full bloom stage. This suggested that DNA reduplication observed in ‘Giant Laf’ fruit is not endoreduplication, but endomitosis or nuclear fusion. To identify genes expressed differentially between in ‘Giant Laf’ and in ‘La France’, microarray analysis was performed with RNA from receptacle (fruit flesh) at 1 week before full bloom stage. To isolate the receptacle, laser microdissection was applied. Genes encoding proteins localized in nucleus and cytoskeleton were up-regulated in ‘Giant Laf’. Among these genes, we found several genes which shared homology with previously described DNA reduplication related genes. These are candidates of responsible gene of ‘Giant Laf’ mutation.

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Comparison of pear M-bM-^@M-^XLa FranceM-bM-^@M-^Y and its bud mutant M-bM-^@M-^XGiant LafM-bM-^@M-^Y (2010 expt, receptacle at 1 week before full bloom stage)

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2012-12-31 | E-GEOD-38550 | biostudies-arrayexpress

Multiple-omics studies of pear fruit flesh

Project description:A comprehensive analysis of the metabolome, proteome, transcriptome, DNA methylome, and small RNAome profiles of pear fruit flesh at 11 developing stages, spanning from fruitlet to ripening.

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Effects of Bloom RNAi on Transcriptomes in A549 Cells

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Increased levels of IAA are required for system 2 ethylene synthesis causing fruit softening in peach (Prunus persica (L). Batsch)

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