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An evolutionarily ancient transcription factor drives spore morphogenesis in mushroom-forming fungi

ABSTRACT: Sporulation is the most widespread means of reproduction and dispersal in fungi. In the Basidiomycota, sexual spores are born on specialised cells, basidia, from which they are discharged forcibly by a powered process called ballistospory, the highest known acceleration in nature. However, the genetics of sporulation, in particular postmeiotic events related to morphogenesis and ballistospory, remain poorly known. Here, we provide novel insights into these processes, through the characterization of a novel, highly conserved transcription factor, SRR1, and its putative downstream regulatory network. Reverse genetics of Srr1 in the model mushroom Coprinopsis cinerea and commercially produced oyster mushroom resulted in sporulation problems, indicating the conserved role of Srr1 across Agaricomycetes. RNA-Seq and motif-based inference of a gene regulatory network of SRR1 allowed delimiting putative targets regulated by SRR1 in a direct and indirect manner. Using this network, and comparative genomics, we identified genes associated with ballistospory, including a putative SRR1-target chitinase, which was found to be required for spore production and morphology. Overall, our study offers new insights into the genetics of postmeiotic spore morphogenesis and ballistospory of Agaricomycetes.

ORGANISM(S): Coprinopsis cinerea

PROVIDER: GSE269820 | GEO | 2025/02/26

REPOSITORIES: GEO

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