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Gene expression in Achilles tendon cells of SD rats after 1 h of running followed by 12 h of rest

ABSTRACT: Achilles tendinopathy is often thought to be a consequence of overuse of the Cells within the Achilles tendon of healthy rats undergo a series of changes following physiologic levels of mechanical stimulation after running, and we further explored the transcriptome changes in Achilles tendon cells during post-exercise recovery. Our current experiment reveals RNA-seq analysis of the transcriptome of the rat Achilles tendon after 12 hours of rest following running.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE270400 | GEO | 2024/10/07

REPOSITORIES: GEO

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Effects of running for 1h on gene expression in Achilles tendon cells of SD rats

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Project description:Tendon is a highly aligned connective tissue, in which the macro-structure consists of collagen-rich fascicles surrounded by interfascicular matrix (IFM). In a series of recent studies in equine tissue, we have demonstrated specialisation of tendon composition, structure and mechanics to achieve the tendon’s functional requirements, specifically reporting extensive specialisation of the IFM region in the energy storing superficial digital flexor tendon. We have also demonstrated loss of functional specialisms with ageing, leading to a hypothesised new paradigm for tendinopathy, focused on the importance of the IFM. However, to date, there have been no studies focused on structure-function specialisation or the IFM in functionally distinct human tendons. Here, we compare the positional anterior tibialis tendon and energy storing Achilles tendon, performing a detailed analysis of the composition and mechanical properties of both fascicle and IFM regions, to test the hypothesis that the IFM in the energy storing Achilles tendon has specialised composition and mechanical properties, and that these specialisations are lost with ageing. We demonstrate that the IFM is specialised in the energy storing Achilles tendon, with greater elasticity and fatigue resistance than in the positional anterior tibialis tendon. While there were few age-related alterations in mechanics, we did identify age-related alterations in the IFM proteome of the Achilles tendon specifically, which is predicted to be regulated by TGF-beta signalling and may be responsible for the trend towards decreased fatigue resistance observed in the Achilles IFM with ageing.

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Effects of running for 1h on gene expression in Achilles tendon cells of SD rats

Project description:Effects of running for 1h on gene expression in Achilles tendon cells of SD rats

| PRJNA1122201 | ENA

Gene expression in Achilles tendon cells of SD rats after 1 h of running followed by 12 h of rest

Project description:Gene expression in Achilles tendon cells of SD rats after 1 h of running followed by 12 h of rest

| PRJNA1126305 | ENA

Expression data from rat Achilles tendon-derived stem cells during development

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Expression data from rat Achilles tendon-derived stem cells during development

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