Transcriptomics

Dataset Information

0

Effectiveness of low-intensity exercise in mitigating active arthritis exacerbation in a mouse rheumatoid-arthritis model


ABSTRACT: Objective To investigate the effects of low-intensity exercise on active arthritis in a SKG mouse model of human rheumatoid arthritis (RA) pathology. Methods Twenty-four female SKG mice were divided into three groups: sedentary (control), AR (induced arthritis), and AREx (induced arthritis plus low-intensity exercise). Arthritis was induced via intraperitoneal administration of mannan. After a 2-week inflammation period, low-intensity treadmill exercise was performed only in the AREx group. Arthritis was assessed weekly during the rearing period. After 4 weeks of exercise, histological and bone morphometric analyses of the right ankle joint were performed. A histological analysis of the gastrocnemius muscle was also performed. Bulk mRNA sequencing was conducted on the left synovial membrane-fat pad (SM-FP) complex. Results The synovitis score showed no change; however, the arthritis score was significantly lower in the AREx group than in the AR group (p<0.05), indicating that low-intensity exercise suppressed arthritis exacerbation. The calcaneal and talar bone volumes decreased in the AR group, whereas the AREx group showed no significant change. In the SM-FP complex tissue, the gene expression of inflammatory cytokines decreased in the AREx group compared with the AR group, particularly the suppression of IL6/Jak/Stat3. Immunohistochemical analysis revealed significantly decreased expression of pro-inflammatory cytokines and increased expression of anti-inflammatory cytokines in the synovium of the AREx group compared with the AR group (p<0.05). Conclusion Low-intensity exercise therapy for active RA showed anti-inflammatory and suppressive effects on arthritis exacerbation in SKG mice, a mouse model of human RA pathology.

ORGANISM(S): Mus musculus

PROVIDER: GSE271058 | GEO | 2025/01/31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA1129519 | ENA
2015-05-14 | E-GEOD-48278 | biostudies-arrayexpress
2024-10-02 | GSE271209 | GEO
2024-04-05 | GSE185577 | GEO
2021-02-16 | GSE166747 | GEO
2021-02-16 | GSE166746 | GEO
2023-08-01 | GSE226072 | GEO
2024-09-02 | BIOMD0000000550 | BioModels
2024-09-02 | BIOMD0000000549 | BioModels
2010-06-08 | E-GEOD-22046 | biostudies-arrayexpress