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Data-driven identification of small molecules inducing Brown Adipocyte differentiation

ABSTRACT: Promoting the recruitment and function of human brown adipose tissue (BAT) holds significant promise for preventing and treating obesity and related metabolic complications. While identifying genes to enhance BAT recruitment and thermogenic activation is crucial, traditional upregulation methods often lack efficiency. Small molecules, however, can effectively facilitate this process through safe and rapid mechanisms. In our study, we employed the DECCODE method, an unbiased drug-induced transcriptomics approach, to identify small molecules that facilitated cell conversion. We began by establishing a transcriptomic profile of BAT cells as our target and then assessed small molecules from the LINCS collection for their similarity to this target, thus identifying a pool of candidates. Our validation in human thermogenic adipocytes revealed that three drugs—Saxagliptin, Tolvaptan, and Targinine—already approved for other uses significantly enhance BAT recruitment and activation in a human thermogenic adipocyte cellular model.

ORGANISM(S): Homo sapiens

PROVIDER: GSE271635 | GEO | 2024/07/08

REPOSITORIES: GEO

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