Dataset Information

Conditioned Extracellular Vesicles Derived from Dedifferentiated Fat Cells Promote Bone Regeneration by Altering MicroRNAs

ABSTRACT: The identification of suitable seed cells represents a critical scientific challenge in the field of oral and maxillofacial bone tissue regeneration. Dedifferentiated fat (DFAT) cells have biological characteristics similar to adipose-derived stem cells (ASCs), but possess the advantages of minimally invasive acquisition, strong proliferation and high homogeneity. Compared to cell therapy, extracellular vesicles (EVs) have lower immunogenicity, better stability and more convenient transportation and storage, which makes EV-based cell-free therapy to be a promising alternative strategy. It is not clear whether EVs derived from DFAT cells can be used as an effective therapy for bone regeneration. The purpose of this study was to verify the effect of EVs derived from DFAT cells on promoting osteogenic differentiation of bone marrow derived mesenchymal stem cells (BMSCs) to provide a reference for the treatment of alveolar bone loss.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE271743 | GEO | 2025/01/08

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:BACKGROUND: Ischemia/reperfusion injury (IRI) is the leading cause of acute kidney injury (AKI). The current standard of care focuses on supporting kidney function, stating the need for more efficient and targeted therapies to enhance repair. Mesenchymal Stromal Cells (MSCs) and their secretome, either as conditioned medium (CM) or extracellular vesicles (EVs), have emerged as promising options for regenerative therapy, however, their full potential in treating AKI remains unknown.METHODS: In this study, we employed an in vitro model of chemically-induced ischemia using antimycin A combined with 2-deoxy-D-glucose to induce ischemic injury in proximal tubule epithelial cells. Afterwards, we evaluated the effects of MSC secretome, CM or EVs obtained from adipose tissue, bone marrow and umbilical cord, on ameliorating the detrimental effects of ischemia. To assess the damage and treatment outcomes, we analyzed cell morphology, mitochondrial health parameters (mitochondrial activity, ATP production, mass and membrane potential) and overall cell metabolism by metabolomics.RESULTS: Our findings show that ischemic injury caused cytoskeletal changes confirmed by disruption of the F-actin network, energetic imbalance as revealed by a 50% decrease in the oxygen consumption rate, increased oxidative stress, mitochondrial dysfunction and reduced cell metabolism. Upon treatment with MSC secretome, the morphological derangements were partly restored and ATP production increased by 40-50%, with umbilical cord-derived EVs being most effective. Furthermore, MSC treatment led to phenotype restoration as indicated by an increase in cell bioenergetics, including increased levels of glycolysis intermediates, as well as an accumulation of antioxidant metabolites.CONCLUSION: Our in vitro model effectively replicated the in vivo-like morphological and molecular changes observed during ischemic injury. Additionally, treatment with MSC secretome ameliorated proximal tubule damage, highlighting its potential as a viable therapeutic option for targeting AKI.

Dataset Information

Conditioned Extracellular Vesicles Derived from Dedifferentiated Fat Cells Promote Bone Regeneration by Altering MicroRNAs

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets