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FOXQ1 Regulates Mitochondrial Cristae Formation and Energy Metabolism in Mouse Brain Endothelial Cells [RNA-seq]

ABSTRACT: Purpose: To gain futher insight into how FOXQ1 regulates the brain endothelial cells ,RNA-seq was used to analyze the genome-wide changes resulting from the cerebral cortical endothelial cells of E18 FOXQ1 conditional knock out mice and littermate wild-type. Methods: Total RNA from E18 Flow sorting of endothelial cells of wild-type(WT) and FOXQ1fl/fl;Tie2-Cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, total RNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 6000 platform in Annoroad Genomics Approximately one thousand transcripts showed differential expression between the wild-type(WT) and FOXQ1fl/fl;Tie2-Cre mice cortical endothelial cells, with a fold change ≥1.5 and p value <0.05. These results reflected FOXQ1 plays a role in brain endothelial cells. Conclusions: RNA-seq based transcriptome characterization would provide a overall understanding how FOXQ1 works in endothelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE271827 | GEO | 2024/07/16

REPOSITORIES: GEO

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