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Tracing the lineage of replicative RNAs in the process of KRAS (S17N) evolution

ABSTRACT: A dominant-negative gene therapy approach has been proposed and tested on proto-oncogene KRAS, wherein the oncogenic activity (and cell proliferation) of KRAS can be suppressed by introducing a dominant-negative KRAS allele (S17N). We employed REPLACE to conduct continuous evolution on KRAS (S17N) and examined its potential pathways for conferring resistance in this gene therapy methodology.To explore the accumulation of mutations in various RNAs during the KRAS (S17N) evolution experiment, we established a barcoded library and conducted lineage tracing of replicative RNAs carrying KRAS (S17N) throughout the evolution process.

ORGANISM(S): Mesocricetus auratus

PROVIDER: GSE271874 | GEO | 2024/09/30

REPOSITORIES: GEO

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