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Blue‐shifting green fluorescent proteins using REPLACE

ABSTRACT: In the directed evolution experiments of EGFP or StayGold, the objective was to blue-shift the excitation light spectrum of GFP towards shorter wavelengths, resulting in an enhancement of fluorescence intensity upon 405 nm laser excitation. This facilitates the advancement of novel fluorescent proteins. These experiments represent a category of experiments that utilize REPLACE to achieve discontinuous directed evolution through FACS sorting (screening).

ORGANISM(S): Mesocricetus auratus

PROVIDER: GSE271876 | GEO | 2024/09/30

REPOSITORIES: GEO

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