RNA-Seq shows that HAVCR1 knockdown does not affect JEG3 interferon signaling response
Ontology highlight
ABSTRACT: To investigate whether HAVCR1 knockout (KO) affects the response of the interferon signaling pathway in JEG3 cells, RNA-Seq was performed on HAVCR1 KO and control JEG3 cells under both basal conditions and stimulation with double-stranded RNA mimic poly I:C. The results indicated that HAVCR1 knockdown does not affect the interferon signaling pathway response in JEG3 cells. Both groups exhibited comparable activation of the interferon signaling pathway.
Project description:In this experiment we compared total RNA from two commonly used choriocarcinoma cell lines, JEG3 and BeWo, to identify differentially expressed transcripts. Keywords: other
Project description:A comparison between a human placental cell line (3A sub E placental, aka, PLC), human choriocarcinoma methotrexate-sensitive JEG3, and methotrexate-resistant JEG3R. This comparison focused on genomic 'hot spot' regions that are frequently mutated in human cancer genes.
Project description:In this experiment we compared total RNA from two commonly used choriocarcinoma cell lines, JEG3 and BeWo, to identify differentially expressed transcripts. GSM48273 CEL file is absent
Project description:Purpose: The goals of this study are to compare transcriptomic profiles of JEG-3 cells in EPS15 knockdown and control cells. Methods: EPS15 scramble oligos were used as a negative control, while an siRNA targetting EPS15 was used to generate knockdown cell lines. Results: Using an optimized data pipeline, we quantified mRNA expression of 37,788 genes and detected differentially-expressed genes across knockdown and control cell lines. Conclusions: Our results provide some experimental evidence that EPS15 has transcriptomic consequences in human placenta-derived JEG3 cells.
Project description:B cell receptor (BCR) signaling and oncogenic tyrosine kinases that mimic BCR-signaling in B-lineage leukemia and lymphoma depend on assembly of membrane proximal signaling complexes. Signalosomes in normal BCR- and oncogene (e.g. BCR-ABL1, RAS-pathway lesions) signal transduction are recruited to phospholipid anchors in lipid rafts. The robustness of these complexes depends on cholesterol accumulation in lipid rafts. Here we identified the interferon-induced transmembrane protein IFITM3 as a central regulator of cholesterol in lipid rafts.
Project description:Transcriptional profiling of human monocyte-derived dendritic cells (MDDCs), comparing cells transfected with miR-29a mimic, or miR-29b mimic, or negative control.