Transcriptomics

Dataset Information

0

RNA-seq in SREBF1-deficient human iPSCs, hiPSC-NSCs and differentiated cells.


ABSTRACT: Omics analyses and qRT-PCR time-course during the transition from hiPSC to hiPSC-NSC highlighted the up-regulation of SREBF1, a gene involved in cholesterol biosynthesis and lipid homeostasis, suggesting its potential role in NSC commitment/maintenance. To test this hypothesis, we generated SREBF1-deficient hiPSC lines by co-delivering ribonucleoprotein Cas9 with a pool of sgRNA targeting exon 5 of the SREBF1 gene. Upon isolation of three clones harboring the deletion we performed RNA-seq analysis in hiPSC, hiPSC-NSC and differentiated cultures at 7 and 14 days of differentiation, compared to control cells. This analysis will allow to identify the potential role of SREBF1 in affecting hiPSC-to-hiPSC-NSC transition, hiPSC-NSC maintenance and commitment toward differentiated cell populations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE273125 | GEO | 2024/09/23

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-09-23 | GSE239444 | GEO
2024-09-23 | GSE239443 | GEO
2024-07-01 | GSE238206 | GEO
2023-10-24 | PXD039817 | Pride
2013-02-28 | E-GEOD-40750 | biostudies-arrayexpress
2013-02-28 | E-GEOD-40749 | biostudies-arrayexpress
2013-02-28 | GSE40749 | GEO
2013-02-28 | GSE40750 | GEO
2021-04-13 | GSE171966 | GEO
2021-01-03 | GSE108752 | GEO