Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide rearrangement of protein translation levels for cell and organelle proliferation in a simple unicellular alga

ABSTRACT: Cell proliferation is a fundamental characteristic of organisms, driven by the holistic functions of multiple proteins encoded in the genome. However, the individual contributions of thousands of genes and the millions of protein molecules they express to cell proliferation are still not fully understood, even in simple eukaryotes. Here, we present proteome maps of cells during proliferation in the unicellular alga Cyanidioschyzon merolae, based on the sequencing of ribosome-protected messenger RNA (mRNA) fragments. Ribosome footprint profiling of genes encoded by nuclear, mitochondrial, and chloroplast DNAs has revealed qualitative and quantitative changes in mRNA transcription and protein translation within each gene during cell division. Comparisons of ribosome footprints from non-dividing and dividing cells allowed the identification of proteins involved in cell proliferation. Given that in vivo experiments on two selected candidate proteins identified a division-phase-specific mitochondrial nucleoid protein and a mitochondrial division protein, these results offer key insights into the comprehensive protein functions that facilitate cell and organelle division.

ORGANISM(S): Cyanidioschyzon merolae

PROVIDER: GSE273424 | GEO | 2025/01/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Isolated peroxisome-division machinery

Project description:LC-MS/MS analysis was performed to determine enriched protein in the fraction of isolated peroxisome-division machinery. Fraction was acquired from uni-cellular red algae Cyanidioschyzon merolae during mitotic phase which contains dividing peroxisome.

2018-09-21 | PXD010716 | JPOST Repository

Effect of PRC2 loss of function on gene and TE expression in Cyanidioschyzon merolae

Project description:To evaluate role of PRC2 on transcriptional regulation in Cyanidioschyzon merolae, we generated loss of function mutants of a Ez homolog in Cyanidioschyzon merolae by homologous recombination based mutagenesis. Then we performed comparative transcriptome analyses using data obtained from Cme(z) and WT.

2023-09-21 | GSE221632 | GEO

RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae

Project description:Light is one of the main environmental cues that affects the physiology and behavior of many organisms. The effect of light on genome-wide transcriptional regulation has been well-studied in green algae and plants, but not in red algae. Cyanidioschyzon merolae is used as a model red algae, and is suitable for studies on transcriptomics because of its compact genome with a relatively small number of genes. In addition, complete genome sequences of the nucleus, mitochondrion, and chloroplast of this organism have been determined. Together, these attributes make C. merolae an ideal model organism to study the response to light stimuli at the transcriptional and the systems biology levels. Previous studies have shown that light significantly affects cell signaling in this organism, but there are no reports on its blue light- and red light-mediated transcriptional responses. We investigated the direct effects of blue and red light at the transcriptional level using RNA-seq. Blue and red light were found to regulate 35% of the total genes in C. merolae. Blue light affected the transcription of genes involved protein synthesis while red light specifically regulated the transcription of genes involved in photosynthesis and DNA repair. Blue or red light regulated genes involved in carbon metabolism and pigment biosynthesis. Overall, our data showed that red and blue light regulate the majority of the cellular, cell division, and repair processes in C. merolae. Identification of blue light and red light regulated genes by deep sequencing in biological duplicates. qRT-PCR was performed to verify the RNA-seq results.

2016-09-01 | E-GEOD-67303 | biostudies-arrayexpress

Transcriptional response of the extremophile red alga Cyanidioschyzon merolae to changes in CO2 concentrations

Project description:Cyanidioschyzon merolae (C. merolae) is an acidophilic red alga growing in a naturally low carbon dioxide (CO2) environment. Although it uses a ribulose 1,5-bisphosphate carboxylase/oxygenase with high affinity for CO2, the survival of C. merolae relies on functional photorespiratory metabolism. In this study, we quantified the transcriptomic response of C. merolae to changes in CO2 conditions. We found distinct changes upon shifts between CO2 conditions, such as a concerted up-regulation of photorespiratory genes and responses to carbon starvation. We used the transcriptome data set to explore a hypothetical CO2 concentrating mechanism in C. merolae, based on the assumption that photorespiratory genes and possible candidate genes involved in a CO2 concentrating mechanism are co-expressed. A putative bicarbonate transport protein and two α-carbonic anhydrases were identified, which showed enhanced transcript levels under reduced CO2 conditions. Genes encoding enzymes of a PEP-CK-type C4 pathway were co-regulated with the photorespiratory gene cluster. We propose a model of a hypothetical low CO2 compensation mechanism in C. merolae integrating these low CO2-inducible components.

2017-07-31 | GSE100372 | GEO

Transcriptomic analysis in C. merolae

Project description:RNA-seq experiment in wildtype Cyanidioschyzon merolae cultures. The aim of the experiment was the comparison of the transcriptomic activity with H3K27me3 profile. The results revealed a negative correlation between expression and H3K27me3 occupancy. The experiment contains 3 biological replicates.

2017-04-01 | GSE93912 | GEO

Temperature induced remodeling of the photosynthetic machinery of the red alga Cyanidioschyzon merolae

Project description:Cyanidioschyzon merolae is a thermophilic red alga with an optimum growth temperature of 42°C. In this study we investigated the acclimation process of the alga to a colder temperature (25°C). To this aim we performed quantitative proteomic analyses of whole cells as well as solubilized thylakoid protein complexes.

2017-04-03 | PXD005615 | Pride

RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae

2016-09-10 | GSE83828 | GEO

RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae

2016-09-01 | GSE67303 | GEO

Identification of proteins from vacuoles of red algae

Project description:Vacuoles and lysosomes are single-membrane-bound organelles involved in diverse functions such as intracellular digestion and storage or secretion of metabolites. To understand their origin, evolution and fundamental features, the identification of proteins comprising these compartments in missing links would be invaluable. So, we isolated the vacuoles from Cyanidioschyzon merolae, which is considered to be one of the most primitive photosynthetic eukaryotes, and identified 46 proteins by matrix-assisted laser desorption/ionization time of flight-mass spectrometry. Keywords: peptide mass fingerprinting, MALDI-TOF

2009-09-01 | GSE12042 | GEO