Reduction of dietary glycemic load modifies expression of several miRNAs associated with energy balance and cancer pathways in premenopausal women
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE27474 | GEO | 2011/12/19
SECONDARY ACCESSION(S): PRJNA138449
REPOSITORIES: GEO
Similar Datasets
Project description:We assessed the effect of dietary glycemic load on miRNA expression in a sample of healthy, premenopausal women participating in a 12 month intervention designed to lower dietary glycemic load. Comparing post-intervention to baseline miRNA expression data of 14 participants receiving the active intervention.
2011-12-19 | E-GEOD-27474 | biostudies-arrayexpress
Project description:Prostate Microarrays for two studies on Low-Fat, Low-Glycemic Load Diet intervention in prostate cancer and the effect of Surgical Manipulation on Prostate Gene Expression. Influence of Surgical Manipulation on Prostate Gene Expression: Implications for Molecular Correlates of Treatment Effects and Disease Prognosis "Measurements of tissue gene expression are increasingly used for disease stratification, clinical trial eligibility, and assessment of neoadjuvant therapy response. However, the method of tissue acquisition alone could significantly influence the expression of specific transcripts or proteins. This study examines whether there are transcript alterations associated with surgical resection of the prostate gland by radical retropubic prostatectomy." Low-Fat, Low-Glycemic Load Diet and Gene Expression in Human Prostate Epithelium: A Feasibility Study of Using cDNA Microarrays to Assess the Response to Dietary Intervention in Target Tissues "We examined the feasibility of using gene expression changes in human prostate epithelium as a measure of response to a dietary intervention." Keywords: Low-fat, Low-Glycemic Load, Prostate Cancer, Radical Prostatectomy, Ischemia
2008-12-01 | GSE10028 | GEO
Project description:Background: Dietary patterns low in glycemic load are associated with reduced risk of cardiometabolic diseases. Improvements in serum lipid concentrations may play a role in these observed associations. Objective: We investigated how dietary patterns differing in glycemic load affect a clinical lipid panel and plasma lipidomics profiles. Methods: In a crossover, controlled feeding study, 80 healthy participants (n=40 men, n=40 women), 18-45 y were randomized to receive low-glycemic load (LGL) or high glycemic load (HGL) diets for 28 days each with at least a 28-day washout period between controlled diets. Fasting plasma samples were collected at baseline and end of each diet period. A clinical lipid panel including total-, VLDL-, LDL-, and HDL-cholesterol and triglycerides were measured using an auto-analyzer. Lipidomics analysis using mass-spectrometry provided the concentrations of 863 species. Linear mixed models were used to test for a diet effect. Results: Lipids from the clinical panel were not significantly different between diets. Lipidomics analysis showed that 67 lipid species, predominantly in the triacylglycerol class, differed between diets (FDR<0.05). A majority of these were higher after the LGL diet compared to the HGL. Conclusion: While the clinical lipid measures did not differ between diets, some lipid species were higher after the LGL diet in the lipidomics analysis. The two diets were eucaloric and had similar percentage of energy from carbohydrate, protein and fat. Thus, the difference in macronutrient, particularly carbohydrate, quality of the LGL diet is likely affecting the composition of lipid species.
2020-09-10 | ST001490 | MetabolomicsWorkbench
Project description:We previously reported that a low versus high glycemic index (GI) diet on a high fat (30% kcal fat) background (LGI and HGI, respectively) significantly retarded adverse health effects in C57BL/6J male mice. The LGI diet enhanced whole body insulin sensitivity and repressed high fat diet-induced body and adipose tissue weight gain, resulting in reduced serum leptin and resistin levels (Faseb J 2009; 23: 1092-1101). How white adipose tissue (WAT) is effected is examined in the present study. We characterized the molecular mechanisms underlying the GI-mediated effects in WAT using whole genome transcriptomics technology. We show that a LGI vs. HGI diet mainly exerts its beneficial effects on substrate metabolism, especially insulin signaling of fatty acid metabolism. In addition, cell adhesion and cytoskeleton remodeling showed reduced expression in line with lower WAT mass, but it might also be due to altered insulin sensitivity. An important transcription factor showing enhanced expression is PPARgamma. Furthermore, serum levels of triglycerides, total cholesterol, HDL- and LDL-cholesterol were significantly reduced by a LGI vs. HGI diet, and muscle insulin sensitivity was significantly increased as analyzed by PKB/Akt phosphorylation. Cumulatively, even though these mice were fed a high fat diet, the low versus high GI induced significantly favorable changes in metabolism in WAT. These effects suggest a partial overlap with pharmacological approaches by thiazolidinediones (TZDs) to treat insulin resistance and statins and plantsterols/stanols for hypercholesterolemia. It is therefore tempting to speculate that such a dietary approach might beneficially support pharmacological treatment of insulin resistance or hypercholesterolemia in humans. We analyzed 19 epididymal whie adipose tissue (epiWAT) samples from a 13 week High fat diet, Low glycemic index dietary group (LGI, n=9) versus a High fat diet, High glycemic index dietary group (HGI, n=10) after 13 weeks of feeding wildtype C57BL/6J male adult mice. Of the 19 arrays, we excluded 2 arrays for downstream analysis based on quality control (total final set contains 8 LGI and 9 HGI samples).
2011-09-12 | E-GEOD-26930 | biostudies-arrayexpress
Project description:Reduction of dietary glycemic load modifies expression of several miRNAs associated with energy balance and cancer pathways in premenopausal women
| PRJNA138449 | ENA
Project description:Full-term pregnancy (FTP) at an early age confers long-term protection against breast cancer, in this study, we evaluated gene expression changes induced by parity in the breast of premenopausal women. Gene expression profiling of normal breast tissue from 30 nulliparous (NP) and 79 parous (P) premenopausal volunteers were performed using Affymetrix microarrays. In addition to a discovery/validation analysis, we conducted analysis of gene expression differences in P vs. NP women as a function of time since last FTP.
2019-03-19 | GSE112825 | GEO
Project description:High glycemic index (HGI) diet induces hyperglycemia, an etiologic risk factor in major diseases affecting multiple organ systems. We hypothesized that adaptation to dietary glycemic exposure is tissue-specific and depends on the length of intake. We therefore evaluated transcriptomic responses to HGI diet after one or 12-months in the liver and retina of C57BL/6J wild-type mice. In the liver, the genes associated with inflammation and fatty acid metabolism were altered within one-month of HGI diet, whereas 12-month HGI diet-fed group showed dysregulated expression of multiple cytochrome P450 genes and overexpression of key lipogenic factors including Srebf1 and Elovl5. In contrast, retinal transcriptomes exhibited fewer HGI-related changes after one-month, and notable alterations in energy metabolism genes were apparent after 12-months of HGI diet. Fatty acid profiles of liver samples corroborated transcriptomic trends and revealed elevated levels of monounsaturated fatty acid in the HGI group. Conversely, saturated fatty acids and polyunsaturated fatty acids were lower in livers of HGI fed animals, regardless of length of exposure. HGI also resulted in a significant increase in blood low-density lipoprotein (LDL) and overall cholesterol levels. We also uncovered diet-aging interactions affecting expression of mitochondrial oxidative phosphorylation genes in the liver, and inherited disease-associated genes in the retina. Our findings provide new insights into tissue-specific adaptive mechanisms, focusing on the liver and the retina, to dietary hyperglycemia.
2024-02-05 | GSE243843 | GEO
Project description:The aim of the experiment was to identify the change of gene expression in human ovaries between premenopausal women and postmenopausal women based on DNA microarrays analysis. 8 human ovary samples were assembled from premenopausal women (n=4) and from postmenopausal women (n=4), respectively. The active transcription profiles of human ovaries were identified through DNA microarrays. Differentially expressed genes (DEGs) were identified as at least two-fold change with statistical significance. Enrichment of functions and signaling pathways analysis were performed based on Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes database.
2022-08-19 | E-MTAB-11494 | biostudies-arrayexpress
Project description:RNA-sequencing was performed on patient mammary epithelial cell subsets from premenopausal and postmenopausal women undergoing breast reduction surgeries to interrogate transcriptional changes in postmenopausal cells.
2023-12-12 | GSE233308 | GEO
Project description:Background: Premenopausal females are protected against T cell-dependent immune activation and development of angiotensin II (Ang II) hypertension compared to males and postmenopausal females. Therefore, the current study hypothesized that specific CD4+ T cell pathways are regulated by estrogen signlaing and Ang II, and contribute to sex differences in T cell cytokine secretion and renal inflammation. Methods and Results: Transcriptomic profiles of CD4+ T cells isolated from spleens of untreated (Control) and Ang II infused (Ang II) premenopausal C57BL/6 mice were examined via RNA sequencing. Ang II significantly regulated expression of 34 genes, of which 11 (Upregulated: CD163, PRG2, S100A9, MPO, CTSG, NGP, LCN2, S100A8; Downregulated: MARCO, IL1b, HSPA1B) were confirmed by real-time PCR. To determine the effect of estrogen on premenopausal expression of RNAseq identifed genes during Ang II infusion we used two models of estrogen signlaing disruption. Both animal wide knockdown of estrogen receptor a and 4-vinylcyclohexene diepoxide (VCD) induction of menopause, significantly altered expression of RNAseq identified genes. To examine the effect of T cell activation on RNAseq gene expression, CD4+ T cells from control and Ang II treated premenopausal and male mice were stimulated in vitro. Sex differences in T cell RNAseq gene expression were associated with sex differences in supernatant concentration of IL10, IL4, and IFNg and renal IL10, IL4, and IL2 content. Conclusions: These estrogen dependent T cell transcriptomic pathways and sex dependent functional differences in T cell cytokine secretion identify novel pathways that may mediate sex specific inflammatory responses during hypertension development.
2020-10-03 | GSE158946 | GEO