Ribosome Profiling Reveals Significant Differences in Global Translational vs. Transcriptional Gene Expression Changes During Early Candida albicans Biofilm Formation
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ABSTRACT: Candida albicans, a major human fungal pathogen, can form biofilms on a variety of inert and biological surfaces. C. albicans biofilms allow for immune evasion, are highly resistant to antifungal therapies and represent a significant complication for a wide variety of immunocompromised patients in clinical settings. While transcriptional regulators and global transcriptional profiles of C. albicans biofilm formation have been well-characterized, very little is known about translational regulation of this important C. albicans virulence property. Here, using ribosome profiling, we define the first global translational profile of genes that is expressed during early biofilm development in a human fungal pathogen, C. albicans. We show that C. albicans biofilm formation involves altered translational regulation of gene classes associated with protein synthesis, transport, plasma membrane, cell wall, polarized growth, cell cycle, secretion, and signal transduction. Interestingly, while similar, but not identical, classes of genes showed transcriptional alterations during early C. albicans biofilm development, we observed very little overlap between specific genes that are up-regulated or down-regulated at the translational vs. transcriptional levels. Our results suggest that distinct translational mechanisms, which could potentially be targeted by novel antifungal strategies, play an important role in directing biofilm development of a major human fungal pathogen.
ORGANISM(S): Candida albicans
PROVIDER: GSE274931 | GEO | 2024/12/22
REPOSITORIES: GEO
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