Genomics

Dataset Information

0

MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells.


ABSTRACT: Deficient DNA repair capacity is associated with genetic lesions accumulation and susceptibility to carcinogenesis. MicroRNAs (miRNAs) are small non-coding RNAs that regulate various cellular pathways including DNA repair. Here we hypothesized that the existence of HBV products may interfere with cellular nucleotide excision repair (NER) through microRNA-mediated gene regulation. We found that NER was impaired in HepG2.2.15 cells, a stable HBV-expressing cell line, compared with its parental cell line HepG2. Altered miRNA expression profile, in particular the significant upregulation of miR-192, was observed in HepG2.2.15 cells. Additionally, ERCC3 and ERCC4, two key factors implicated in NER, were identified as targets of miR-192 and over-expressing miR-192 significantly inhibited cellular NER. These results indicated that persistent HBV infection might trigger NER impairment in part through upregulation of miR-192, which suppressed the levels of ERCC3 and ERCC4. It provides new insight into the effect of chronic HBV infection on NER and genetic instability in cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE27561 | GEO | 2011/08/03

SECONDARY ACCESSION(S): PRJNA138399

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-08-02 | E-GEOD-27561 | biostudies-arrayexpress
2011-01-14 | E-GEOD-19980 | biostudies-arrayexpress
2011-01-14 | GSE19980 | GEO
2019-08-24 | GSE119072 | GEO
2022-12-31 | GSE196469 | GEO
2012-01-15 | E-GEOD-19981 | biostudies-arrayexpress
2017-05-24 | GSE81831 | GEO
2017-05-24 | GSE81835 | GEO
2014-11-05 | GSE62951 | GEO
2012-01-15 | GSE19981 | GEO