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Multiomic single-cell sequencing of skin and blister fluid from patients with severe cutaneous adverse drug reactions.

ABSTRACT: Severe cutaneous adverse reactions (SCAR) are rare but life-threatening drug reactions mediated by human leukocyte antigen (HLA) class I-restricted CD8+ T-cells. To obtain an unbiased assessment of SCAR cellular immunopathogenesis, we performed single-cell (sc) transcriptome, surface proteome, and TCR sequencing (5' scRNA-TCR-CITE-seq, 10x Genomics) on unaffected skin, affected skin, and blister fluid from diverse SCAR patients.

ORGANISM(S): Homo sapiens

PROVIDER: GSE275871 | GEO | 2024/09/18

REPOSITORIES: GEO

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