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A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenic effectors (total and polyA RNA-Seq)

ABSTRACT: Small nucleolar RNAs (snoRNAs) are non-coding transcripts that guide chemical modifications of RNA substrates and contribute to gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA (lncRNA) mamRNA and carrying two independent cellular functions. On one hand, snR107 guides site-specific 25S rRNA 2’-O-methylation and promotes pre-rRNA processing/60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2’-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE276243 | GEO | 2025/03/17

REPOSITORIES: GEO

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A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenic effectors (meiosis RNA-Seq)

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