Transcriptomics

Dataset Information

0

Differential transcript accumulation in seedling and mature shoots identifies components contributing to corn earworm resistance


ABSTRACT: As maize seedlings germinate into the soil, they encounter an environment teeming with insects seeking rich sources of nutrition. Maize presumably has developed a number of molecular mechanisms to ensure survival at the beginning of its life cycle. Bioassays indicated maize seedlings were more toxic to caterpillars than shoots from 3 or 4 leafed plants. Microarray technology was utilized to document the expression of a number of genes with potential defensive functions in seedling tissue. In addition to elevated levels of the genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), an anti-insect resistance molecule, other highly expressed genes in the seedling encode the following putative proteins: defensin, hydroxyproline and proline-rich protein, thaumatin-like protein, lipase, cystatin, protease inhibitor, and a variety of proteases. Changes in the levels of gene expression by the microarray technology were well correlated to results using quantitative RT-PCR. The potential resistance genes identified occurred mainly on chromosomes 1 and 5 in the B73 genome. Analysis of promoters of four DIMBOA biosynthetic genes determined that a Dof transcription factor, two of which were differentially expressed in three vegetative stages, is possibly involved in regulation of the DIMBOA biosynthetic pathway. The results indicate that maize employs a wide variety of potential resistance mechanisms in seedling tissue to resist a possible insect attack.

ORGANISM(S): Zea mays

PROVIDER: GSE27709 | GEO | 2011/11/03

SECONDARY ACCESSION(S): PRJNA138117

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-11-03 | E-GEOD-27709 | biostudies-arrayexpress
2015-10-05 | GSE62287 | GEO
2012-12-01 | GSE42659 | GEO
2011-10-01 | GSE28244 | GEO
2012-08-16 | GSE40107 | GEO
2011-09-30 | E-GEOD-28244 | biostudies-arrayexpress
2015-02-04 | GSE54166 | GEO
2012-12-03 | GSE40952 | GEO
2019-11-19 | GSE140562 | GEO
2011-11-22 | GSE33391 | GEO