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Effect of enhanced early life nutrition on hypothalamic arcuate nucleus miRNA transcriptome in bull calves.

ABSTRACT: The aim of this study was to measure the impact of contrasting feeding regimes in the first 12 wk of life, on the molecular control of hypothalamic arcuate nucleus tissue in bull calves. Holstein bull calves were designated to high (HI; n=15) or moderate (MOD; n=12) dietary groups, with diets designed to provoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, all calves were euthanized, and hypothalamic arcuate nucleus tissue harvested. RNA was extracted from the hypothalamic arcuate nucleus and used for miRNAseq.

ORGANISM(S): Bos taurus

PROVIDER: GSE277781 | GEO | 2025/04/22

REPOSITORIES: GEO

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Effect of enhanced early life nutrition on the hypothalamic arcuate nucleus transcriptome of bull calf

Project description:This study evaluated the effect of enhanced dietary intake during the early life period on hypothalamic arcuate nucleus transcriptome in bull calves. Between 2-12 weeks of age bull calves were offered either a high (HI; n=15) or moderate (MOD; n=15) plane of nutrition, with diets designed to evoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, arcuate nucleus tissue samples were harvested from all calves and subsequently subjected to mRNAseq.

2025-04-22 | GSE278108 | GEO

Effect of enhanced early life nutrition on testes miRNA transcriptome in bull calves

Project description:The aim of this study was to measure the impact of contrasting feeding regimes in the first 12 wk of life, known to impact age at puberty on the molecular control of the testes in bull calves. Holstein bull calves were designated to high (HI; n=15) or moderate (MOD; n=15) dietary groups, with diets designed to provoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, all calves were euthanized, and testes parenchyma harvested. RNA was extracted from the testes and used for miRNAseq. Bioinformatic analysis identified 7 miRNA as differentially expressed between the HI and MOD treatment groups, with target mRNA genes invovled in pathways related to AMPK and IGF-1 signaling.

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Proteomic analysis underlying the impact of early life nutrition on the molecular control of testicular development in the young bull calf.

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Effect of enhanced early life nutrition on the bull calf testes transcriptome

Project description:This study evaluated the effect of enhanced dietary intake during the early life period on testes transcriptome in bull calves. Between 2-12 weeks of age bull alves were offered either a high (HI; n=15) or moderate (MOD; n=15) plane of nutrition, with diets designed to evoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, testes parenchyma tissue samples were harvested from all calves and subsequently subjected to mRNAseq. Subsequent bioinformatics analyses revealed differential expression of genes invovled in cellular adhesion and immune function.

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Effect of enhanced early life nutrition on hypothalamic arcuate nucleus miRNA transcriptome in bull calves.

Project description:Effect of enhanced early life nutrition on hypothalamic arcuate nucleus miRNA transcriptome in bull calves.

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Effect of enhanced early life nutrition on the hypothalamic arcuate nucleus transcriptome of bull calf

Project description:Effect of enhanced early life nutrition on the hypothalamic arcuate nucleus transcriptome of bull calf

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Estrogen Receptor alpha DNA-binding in the arcuate and AVPV nuclei of the hypothalamus in female mice

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2024-12-10 | GSE276414 | GEO

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Project description:The aim of this study was to investigate the effect of early calf-hood nutrition on the transcriptomic profile of the arcuate nucleus of the hypothalamus, the anterior pituitary and the testes in Holstein-Friesian bulls and possible downstream effects on reproduction. Holstein Friesian bull calves with a mean (±S.D.) age and bodyweight of 19 (±8.2) days and 47.5 (±5.3) kg, respectively, were assigned to either a high (n=10) or low (n=10) plane of nutrition. Calves were fed in order to achieve an overall growth rate of 1.2 and 0.5 kg/day for the high and low plane of nutrition treatments, respectively. At 126 days of age, the bulls were euthanized using an intravenous overdose of sodium pentobarbitone and arcuate nucleus of the hypothalamus, anterior pituitary and the parenchyma of the testes tissue samples were harvested and RNAseq analysis was performed. At the end of the trial period,

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