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Spatial mapping of transcriptomic and lineage plasticity in metastatic pancreatic cancer [CosMx]

ABSTRACT: Patients with treatment-refractory pancreatic cancer often succumb to widespread systemic metastases; however, the transcriptomic heterogeneity that underlies recalcitrance to therapy remains understudied, particularly in the spatial context. We constructed high-resolution spatial maps of transcriptional heterogeneity, clonal architecture and lineage plasticity using spatially resolved transcriptomics (SRT) from 13 primary cancers and 36 corresponding liver, lung, and peritoneal metastases, collected via a rapid (“warm”) autopsy program. To validate findings from our SRT dataset at single-cell resolution, we performed CosMX SMI profiling (Nanostring) on 7 samples from 3 patients, including primary and/or liver metastasis.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE277782 | GEO | 2025/02/21

REPOSITORIES: GEO

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