Transcriptomics

Dataset Information

0

Identification of LRP1 and CD13 expressing human periosteal cells for stem cell-mediated bone repair [human scRNA-seq]


ABSTRACT: Human periosteal skeletal stem cells (P-SSCs) are critical for cortical bone maintenance and repair. However, their in vivo identity, molecular characteristics, and specific markers remain unknown. Here, single-cell sequencing revealed human periosteum contains SSC clusters expressing known SSC markers, PDPN and PDGFRA. Notably, human P-SSCs, but not bone marrow SSCs (BM-SSCs), selectively expressed newly identified markers, LRP1 and CD13. Single-cell analysis of mouse periosteum further supported the preferential expression of LRP1 and CD13 in Prx1 P-SSCs. These LRP1CD13 human P-SSCs were perivascular cells with high osteochondrogenic but minimal adipogenic potential. In addition, LRP1CD13 human P-SSCs are maintained in vitro and self-renew in vivo upon transplantation into bone injuries in mice. When Lrp1 was conditionally deleted in Prx1-lineage cells, it led to severe bone deformity, short statue, and periosteal defects. By contrast, local treatment with a LRP1 agonist at the injury sites induced early P-SSC proliferation and bone healing. Thus, human periosteum contains unique osteochondrogenic stem cell subsets, and these P-SSCs express specific markers, LRP1 and CD13, with regulatory mechanism through LRP1 that enhances P-SSC function and bone repair.

ORGANISM(S): Homo sapiens

PROVIDER: GSE278165 | GEO | 2024/11/04

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-11-04 | GSE276574 | GEO
2023-02-22 | GSE218550 | GEO
2018-10-18 | E-MTAB-6417 | biostudies-arrayexpress
2024-10-16 | GSE255137 | GEO
2021-06-11 | GSE161477 | GEO
2024-08-13 | GSE236686 | GEO
2022-02-02 | GSE154003 | GEO
| PRJNA1157865 | ENA
2022-08-10 | GSE195940 | GEO
2024-04-01 | GSE234451 | GEO