Other

Dataset Information

0

CRISPR-CLEAR: Nucleotide-Resolution Mapping of Regulatory Elements via Allelic Readout of Tiled Base Editing


ABSTRACT: CRISPR tiling screens have advanced the identification and characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing. This study introduces CRISPR-CLEAR, an end-to-end experimental assay and computational pipeline, which leverages targeted sequencing of CRISPR-introduced alleles at the endogenous target locus following dense base-editing mutagenesis. This approach enables the dissection of regulatory elements at nucleotide resolution, facilitating a direct assessment of genotype-phenotype effects.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE278581 | GEO | 2024/10/03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA1167867 | ENA
2021-07-26 | E-MTAB-9946 | biostudies-arrayexpress
2015-11-23 | E-GEOD-71763 | biostudies-arrayexpress
2020-05-20 | PXD015702 |
2016-09-01 | E-GEOD-78064 | biostudies-arrayexpress
2021-05-27 | MSV000087529 | MassIVE
2022-03-03 | E-MTAB-11497 | biostudies-arrayexpress
2022-03-05 | E-MTAB-11470 | biostudies-arrayexpress
2021-07-26 | E-MTAB-9937 | biostudies-arrayexpress
2024-10-16 | E-MTAB-13904 | biostudies-arrayexpress