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Mapping the Evolution of Acinar cell derived Pancreatic Preneoplastic Lesions in a Mouse Model: A Detailed Transcriptomic and Pathological Characterization

ABSTRACT: Background & Aims: Pancreatic ductal adenocarcinoma (PDAC) is among the deadliest cancers with poor prognosis and bad therapy outcome. Therefore, mouse models are frequently used to study PDAC and pancreatic preneoplastic lesions. This study aims to evaluate a proposed progression model from acinar cells via acinar-to-ductal metaplasia (ADM) to mucinous tubular complexes (MTC) at transcriptomic level and to investigate the molecular changes behind acinar-cell derived preneoplastic lesions in a mouse model of PDAC. Methods: Spatial transcriptomics was combined with pathological characterization of preneoplastic lesions in p48Cre/+-LSL-KrasG12D/+ mice (n=5) using the 10x Visium® platform. Integration with the Mouse Cell Atlas (MCA) 2.0 single-cell dataset was performed. Bioinformatic analysis was conducted using different Python toolkits and by calculating a pseudotime along the progression axis. Findings were validated at the protein level using immunohistochemistry (IHC). Results: Transcriptional data aligned well with pathological annotations and single-cell data confirmed a decrease in acinar cells and an increase in ductal cells during ADM. Pseudotime analysis supported the progression from acinar cells via ADM to MTC, with marker gene expression patterns along this axis. Upregulation of Keratin 19 and Tetraspanin 8, and downregulation of Amylase 1 and Claudin 10, were observed. Claudin 18 was upregulated specifically in MTC, while Mesothelin and Cathepsin L peaked during ADM. Validation by IHC confirmed the expression of Keratin 19, Amylase 1, Claudin 18 and Mesothelin on protein level. Conclusions: This study confirms the development of MTC from ADM and identifies novel biomarkers along the preneoplastic progression axis in a mouse model of PDAC, advancing our understanding of early pancreatic cancer development.

ORGANISM(S): Mus musculus

PROVIDER: GSE279507 | GEO | 2025/03/11

REPOSITORIES: GEO

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Project description:Pancreatic ductal adenocarcinoma (PDAC) is characterized by desmoplastic stroma surrounding most tumors. Activated stromal fibroblasts, namely Cancer-associated fibroblasts (CAFs), play a major role in PDAC progression. Here we analyzed if CAFs influence acinar cells and impact PDAC initiation, namely “Acinar to Ductal Metaplasia” (ADM). ADM connection with PDAC pathophysiology is indicated but not yet established. Hence, we hypothesized that CAF secretome might play a significant role in ADM in PDAC initiation. Mouse and human acinar cell organoids, acinar cells cocultured with CAFs and exposed to CAF-conditioned media (CAF-CM), acinar cell explants, and CAF cocultures, etc., were examined by qRT-PCR, RNA-seq, immunoblotting and confocal microscopy. Data from LC-MS/MS analysis of CAF CM and RNAseq data of acinar cells post-CM exposure were integrated using bioinformatics tools to identify molecular mechanism for CAF-induced ADM. Using confocal microscopy, immunoblotting, and qRT-PCR analysis, we validated the depletion of key signaling axis in cell-line, acinar explant coculture, and mCAFs. Close association of acino-ductal (UEA1, Amylase, Ck19) markers and mCAFs (α-SMA) in LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx1Cre (KPC) and LSL-KrasG12D/+; Pdx1Cre (KC) autochthonous progression tumor tissue was observed. Caerulein treatment-induced mCAFs increased Ck19 and decreased Amylase in wild-type (WT) and KC pancreas. Likewise, acinar-mCAF cocultures revealed induction of ductal transdifferentiation in cell-line, acinar-organoid, and explant coculture formats in WT and KC mice pancreas. Proteomic and transcriptomic data integration revealed a novel Laminin5/Integrinα4/Stat3 axis responsible for CAF-mediated acinar to ductal cell transdifferentiation. Results collectively suggest the first evidence for CAF-influenced acino-ductal phenotypic switchover, thus highlighting the role of the tumor micro-environment in the inception of pancreatic carcinogenesis.

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Mapping the Evolution of Acinar cell derived Pancreatic Preneoplastic Lesions in a Mouse Model: A Detailed Transcriptomic and Pathological Characterization

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