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Exploring the effect of m6A methylation modification on the progression of bovine ovary tissue

ABSTRACT: Exploring the effect of m6A methylation modification on the progression of bovine ovary tissue before and after the onset of puberty

ORGANISM(S): Bovidae

PROVIDER: GSE279563 | GEO | 2024/11/14

REPOSITORIES: GEO

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Exploring the effect of m6A methylation modification on the progression of bovine hypothalamic tissue

Project description:Exploring the effect of m6A methylation modification on the progression of bovine hypothalamic tissue before and after the onset of puberty

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Transcriptome profiling of m6A mRNA modification in inflammation of bovine mammary epithelial cells infected by Staphylococcus aureus and Escherichia coli

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Project description:N6-methyl-adenosine (m6A) is the most abundant modification on messenger RNAs and is linked to human diseases, but its functions in mammalian development are poorly understood. Here we reveal the evolutionary conservation and function of m6A by mapping the m6A methylome in mouse and human embryonic stem cells. Thousands of messenger and long noncoding RNAs show conserved m6A modification, including transcripts encoding core pluripotency transcription factors. m6A is enriched over 3M-bM-^@M-^Y untranslated regions at defined sequence motifs, and marks unstable transcripts, including transcripts turned over upon differentiation. Genetic inactivation or depletion of mouse and human Mettl3, one of the m6A methylases, led to m6A erasure on select target genes, prolonged Nanog expression upon differentiation, and impaired ESCM-bM-^@M-^Ys exit from self-renewal towards differentiation into several lineages in vitro and in vivo. Thus, m6A is a mark of transcriptome flexibility required for stem cells to differentiate to specific lineages. Examing m6A modification differences in two different cell types

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