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Preconditioning With Electrical Stimulation Promotes Flap Survival by Improving Blood Supply Via ROS/Nrf2/HO1 Pathway

ABSTRACT: We have previously demonstrated that preconditioning flap donor with electrical stimulation (ES) could increase the survival area of pedicled perforator flaps in a rat model. However, the detailed molecular mechanisms remain unclear. In this study, we first verified the effects of ES through in vivo and in vitro experiments. Then we explored the underlying mechanisms in vitro. The results indicated that preconditioning with ES could not only increase the flap survival area but mitigate human umbilical vein endothelial cells (HUVECs) dysfunction. Furthermore, low level reactive oxygen spices (ROS) were produced in HUVECs after ES, serving assignaling molecule to activate the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HOl) pathway. Then ROS scavenger was used to attenuate thegeneration of ROS, resulting that activation of the Nrf2/HOl pathway was inhibited. These results suggest that ROS/Nrf2/HO1 pathway may play pivotal roles in the ES pretreatment induced flap survival.

ORGANISM(S): Homo sapiens

PROVIDER: GSE280048 | GEO | 2024/11/01

REPOSITORIES: GEO

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Preconditioning With Electrical Stimulation Promotes Flap Survival by Improving Blood Supply Via ROS/Nrf2/HO1 Pathway

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