Transcriptomics

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Deep phenotyping of peripheral mononuclear blood cells in health and disease


ABSTRACT: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls and patients with various diseases.. Cells were stained with CD45 and sample tags for identification purposes. CD45+ live cells were enriched through flow sorting and samples were pooled. Samples were then stained with 30 AbSeq antibodies and loaded onto 4 cartridges to account for interplate differences. Pooled sequencing libraries were then sequenced on NovaSeq 6000 (Illumina) using a S2 Reagent Kit v1.5 (200 cycles).

ORGANISM(S): Homo sapiens

PROVIDER: GSE280319 | GEO | 2025/01/06

REPOSITORIES: GEO

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