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Conserved DNA Methylation Signatures in the Prefrontal Cortex of Newborn and Juvenile Guinea Pigs Following Antenatal Betamethasone Exposure

ABSTRACT: We investigated the effect of antenatal corticosteroid exposure (ACS) on the DNA methylation patterns in the prefrontal cortex (PFC) of female guinea pig offspring. Guinea pig dams were treated with saline or betamethasone on gestational days 50/51. PFC was collected from newborn (post-natal day 1) and juvenile (PND 14) offspring. Methylation assessment using genomic DNA was performed with reduced representation bisulfite sequencing techniques.

ORGANISM(S): Cavia porcellus

PROVIDER: GSE280726 | GEO | 2024/11/06

REPOSITORIES: GEO

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Identification of a DNA methylation signature in whole blood of newborn guinea pigs and human neonates following antenatal betamethasone exposure

Project description:We investigated the effect of antenatal corticosteroid exposure (ACS) on the DNA methylation patterns in the whole blood of female guinea pig offspring. Guinea pig dams were treated with saline or betamethasone on gestational days 50/51. Whole blood was collected from newborn offspring (post-natal day 1) and dried on blood spot cards. Methylation assessment using genomic DNA was performed with reduced representation bisulfite sequencing techniques.

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Conserved DNA Methylation Signatures in the Prefrontal Cortex of Newborn and Juvenile Guinea Pigs Following Antenatal Betamethasone Exposure

Project description:Conserved DNA Methylation Signatures in the Prefrontal Cortex of Newborn and Juvenile Guinea Pigs Following Antenatal Betamethasone Exposure

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Identification of a DNA methylation signature in whole blood of newborn guinea pigs and human neonates following antenatal betamethasone exposure

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DNA methylome signatures of prenatal exposure to synthetic glucocorticoids in hippocampus and peripheral whole blood of female guinea pigs in early life

Project description:We examined the hypothesis that prenatal synthetic glucocorticoids (sGC) administration alters DNA methylation signatures in Guinea pig offspring hippocampus and whole blood. Guinea pigs were treated with sGC or saline in late gestation. Genome-wide modifications of DNA methylation were analyzed using reduced representation bisulfite sequencing in juvenile female offspring.

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Antenatal Glucocorticoid Exposure Induced Neurodegenerative Changes in the Fetal Ovine Hippocampal Transcriptome Independent of Pulmonary Immaturity

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Project description:Stress and glucocorticoid exposure during pregnancy alters neurodevelopment and behavior in offspring, and these effects extend multiple generations through a paternal lineage. The epigenetic mechanisms that govern this transgenerational transmission are unclear. We hypothesized that maternal exposure to multiple courses of sGC in late pregnancy would result in altered miRNA levels in germs cells of male guinea pigs across three generations. Further, our behavioral data indicate that F2 females exhibit altered behaviors following sGC exposure. Thus, we evaluated the miRNA profile of F2 female prefrontal cortex to determine the mechanisms responsible for these behavioral changes and to compare these data to our germ cell miRNA analysis. We used miRNA microarray to evaluate the miRNA levels in F1-F3 germ cells and F2 female PFC in guinea pigs that were exposed to control and F0 prenatal sGC exposure. We identified no significant changes to miRNA levels in both F1-F3 germ cells and F2 PFC from guinea pigs in the sGC group.

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2012-10-01 | E-GEOD-32447 | biostudies-arrayexpress

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