Siglecf Deficiency Prevents Fibrosis After Bleomycin-Induced Acute Lung Injury

ABSTRACT: Airway macrophages (AMs) are critical for resolving inflammation and repairing injured lung tissue, but can also contribute to the development of fibrosis. AMs were isolated from bronchoalveolar lavage fluid of Siglec-F KO and WT mice prior to and 7, 14, and 21 days after bleomycin challenge (single dose, 2.5 units/kg, IT). RNA was isolated using the Qiagen micro plus RNA kit and 200 ng of total RNA was used to generate RNA-Seq libraries. The libraries were run on the NovaSeq 6000 with 80 million pair-end reads per sample.

ORGANISM(S): Mus musculus

PROVIDER: GSE280727 | GEO | 2024/12/02

REPOSITORIES: GEO