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Prolonged Crowding Drives Cancer Cell Invasiveness

ABSTRACT: Our study found that prolonged crowding drives cancer cell invasiveness with memory retention. To further characterize gene expression regulated by prolonged crowding, RNA sequencing was performed in crowding-primed cells (20 days) followed by sparse culture for different times (0, 1, 3, or 10 days).

ORGANISM(S): Homo sapiens

PROVIDER: GSE281770 | GEO | 2024/12/01

REPOSITORIES: GEO

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Project description:Prolonged Crowding Drives Cancer Cell Invasiveness

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Project description:All extant amniotes employ a single-layered epithelium of epiblasts as a starting material for gastrulation, suggesting the necessity of the epithelial structure for three germ layer derivation. Using a human embryonic stem cell (hESC) epithelium as a model system, we found that local epithelial crowding derepresses the neuroectoderm fate by spatiotemporal inactivation of ETV4. ETV4 serves as a genetic toggle switch that links cell density to lineage fates. Mechanistically, cell crowding blocks FGF receptor endocytosis by reduced cell-extracellular matrix (ECM) interaction. Disrupted endocytosis decreases ETV4 protein stability by ERK inactivation. Mathematical modeling of epithelial crowding demonstrates that the cooperativity of integrin-ECM interaction transforms the gradient of crowdedness into bistable ETV4 transition, which ensures the switch-like function of ETV4 in lineage determination. Our results propose local cell crowding in a stem cell epithelium as a key cellular mechanism for spatiotemporal regulation of lineage fates.

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Expression analysis of Antirrhinum majus inflorescence meristems grown under control and crowding conditions

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Expression analysis of Antirrhinum majus inflorescence meristems grown under control and crowding conditions

Project description:Investigation of gene expression level changes as a result of crowding growth conditions. A five-chip study using total RNA recovered from samples of inflorescence meristems of Antirrhinum majus cv.165E, grown as single plant per pot (control) and 10 plants per pot (crowded conditions). Each chip measures the expression level of 11,959 ESTs with up to six 60-mer probes per target.

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Project description:Recirculation systems (RAS), which reduce water consumption and improve pathogen control are increasingly used in Atlantic salmon aquaculture. Performance and adaptation of fish to new farming environment is actively investigated. Here, responses to crowding stress were compared in salmon reared in two systems (RAS with brackish water and flowthrough with full salinity water) at low and high density.

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Gene differential expressions between cell subpopulations with high invasiveness (invaded) and low invasiveness (non-invaded) in human lung cancer H460 cell line

Project description:Cell populations with different invasiveness were isolated and collected with cell culture inserts of Boyden-type cell invasion chambers coated with 1xBME (base membrane extract). Cells that invaded to bottom chamber were collected as cell population with high-invasiveness , and cells that remained in the insert chamber were collected as cell population with low-invasiveness. The selection with Boyden-type cell invasion chambers was repeated six times, and cells with high invasiveness were terms as H-INV subline, and cells with low invasiveness were termed as L-INV subline. Microarray analysis was performed with Illumina HumanHT-12 v4 Expression BeadChips

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Microarray-predicted marker genes and molecular pathways indicating combined crowding and thermal stress in rainbow trout (Oncorhynchus mykiss)

Project description:Stress represents a major factor negatively affecting fish welfare in aquaculture. The objective of the present study was to identify and evaluate informative indicators for the welfare of rainbow trout Oncorhynchus mykiss exposed to (A) a critical water temperature of 27 °C and (B) acute crowding of 100 kg/m3 combined with water temperature of 27 °C. In order to make an approximate assessment of the overall condition, we determined health index, spleen-somatic index and haematocrit and recorded the blood concentrations of haemoglobin, cortisol and glucose of rainbow trout under challenging versus control conditions. Moreover, we analysed the transcriptomic profiles of the spleen of the two stress-treatment and the reference groups to identify gene sets, which are specific for temperature stress alone or combined temperature and crowding stress.

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