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RNA-seq of TGF-beta1-induced NRK-52E cells after treatment with 2D- and 3D-EVs

ABSTRACT: Renal tubular epithelial cells are the main cells affected in the process of renal fibrosis, and it has been shown that mesenchymal stem cell-derived exosomes can treat renal fibrosis, and then the traditional 2D-EVs production method is inefficient. We efficiently extracted 3D-EVs by coaxial bioprinting, but the mechanism of 3D-EVs for treating renal fibrosis is not yet known. Therefore, we used TGF-beta1 to induce a renal fibrosis phenotype in NRK-52E cells and took 2D- and 3D-EVs for treatment in order to explore the possible differential molecular mechanisms of 2D- and 3D-EVs for renal fibrosis. This approach facilitates the discovery of new mechanisms for exosomes to treat renal fibrosis.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE281863 | GEO | 2024/12/18

REPOSITORIES: GEO

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Expression data from EMT-derived NRK-52E cells before and after the AMPKα2 knockdown

Project description:Recent studies have shown that AMPKα2 can regulate epithelial-mesenchymal transition(EMT) processes during kidney fibrosis. However, the underlying mechanisms for AMPKα2 changes in renal tubular EMT remain unclear. TGF-β1 was used to induce epithelial-mesenchymal transition(EMT) in normal rat renal tubular epithelial (NRK-52E) cells. Gene microarray was used to analyze differential gene expression in EMT-derived NRK-52E cells before and after the AMPKα2 knockout

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Expression data from NRK-52E cells treated with aristolochic acids for 6h, 24h and 72h

Project description:In this study we have examined the effect of sub-cytotoxic exposure to aristolochic acids (1.65µM) at 6h, 24h and 72h on the whole-genome expression profile in a rat proximal renal tubule cell line (NRK-52E). We used microarrays to detail the mechanism of toxicity and possibly carcinogenicity of aristolochic acids in rat renal proximal cells. NRK-52E cells were cultured to confluence on 6-well plates. Cells were then exposed to aristolochic acid dissolved in DMSO (0.1%) at the IC10 concentration at 72h (1.65 ?M) or DMSO only as control. After 6h, 24h and 72h the medium was removed and RNA was extracted from the cells. Three studies were conducted at each time point.

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Expression data from NRK-52E cells treated with aristolochic acids for 6h, 24h and 72h

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Expression data from NRK-52E cells treated with monuron for 6h, 24h and 72h and rats exposed to monuron for 72h.

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Expression data from NRK-52E cells treated with monuron for 6h, 24h and 72h and rats exposed to monuron for 72h.

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