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Transcriptional changes in PC12 cells induced by repetitive transcranial magnetic stimulation (rTMS) in MSCs-inhibited inflammatory conditions

ABSTRACT: Neuronal death is the primary cause of poor outcomes in cerebral ischemia. The Inflammatory infiltration in the early phase of ischemic stroke plays a vital role in triggering neuronal death. Either transplantation of mesenchymal stem cells (MSCs) derived from humans or repetitive transcranial magnetic stimulation (rTMS) have respectively proved to be neuroprotective and anti-inflammatory in cerebral ischemia. However, either treatment above has its limitations. Whether these two therapies have synergistic effects on improving neurological function and the underlying mechanisms remains unclear.Using an in vitro model of PC12 nerve cell co-culture with MSCs, we demonstrated a transcriptional response pattern, including REST, in which rTMS inhibited PANoptosis of PC12 cells in a low-inflammation environment induced by MSCs. This molecular investigation is helpful in providing a basis for sorting out the complex molecular mechanism of combination of rTMS and MSCs in treating ischemic stroke.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE282396 | GEO | 2024/12/06

REPOSITORIES: GEO

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Project description:Stroke remains a major leading cause of death and disability worldwide. Despite continuous advances, the identification of key molecular signatures of ischemic stroke within the hyper-acute phase of the disease is still of primary interest for a real translational research on stroke diagnosis, prognosis and treatment. High-throughput - omics technologies are enabling large-scale studies on stroke pathology at different molecular levels. Data integration resulting from these -omics approaches is becoming crucial to unravel the interactions among all different molecular elements and highly contribute to interpret all findings in a complex biological context. Here, we have used advanced data integration methods for multi-level joint analysis of transcriptomics and proteomics datasets depicted from the mouse brain 2h after cerebral ischemia. By modeling network-like correlation structures, we identified a set of differentially expressed genes and proteins by ischemia with a relevant association in stroke pathology. The ischemia-induced deregulation of 10 of these inter-correlated elements was successfully verified in a new cohort of ischemic mice, and changes in their expression pattern were also evaluated at a later time-point after cerebral ischemia. Of those, CLDN20, GADD45G, RGS2, BAG5 and CTNND2 were highlighted and evaluated as potential blood biomarkers of cerebral ischemia in blood samples from ischemic and sham-control mice and from ischemic strokes and other patients presenting stroke-mimicking conditions. Our findings indicated that CTNND2 and GADD45G levels in blood within the first hours after ischemic stroke might be potentially useful to discriminate ischemic strokes from mimics and to predict patients’ poor outcome after stroke, respectively. In summary, we have here used for the first time an integrative approach to elucidate by means of biostatistical tools key elements of the initial stages of the stroke pathophysiology, highlighting new outstanding proteins that might be further considered as blood biomarkers of ischemic stroke.

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Transcriptional changes in PC12 cells induced by repetitive transcranial magnetic stimulation (rTMS) in MSCs-inhibited inflammatory conditions

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