Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Infiltration-RNAseq reveals enhanced defense responses in Nicothiana benthamiana leaves overexpressing the banana MaWRKY45 gene

ABSTRACT: Members of the WRKY transcription factor (TF) family play a crucial role in regulating plant defense responses to biotic stress. Here, we report that the constitutive overexpression of the banana MaWRKY45 gene in the leaves of the model plant Nicotiana benthamiana enhances the transcript levels of immune response genes. Among the genes that were up-regulated, we identified those involved in PAMP-triggered immunity, effector-trigger immunity and systemic acquired resistance, among others. These results provide insights into the molecular mechanisms triggered downstream of a single TF such as the banana MaWRKY45. Our findings suggest that MaWRKY45 is a promising candidate for banana breeding programs focused on enhancing disease resistance.

ORGANISM(S): Nicotiana benthamiana

PROVIDER: GSE282538 | GEO | 2025/02/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The transcriptional landscape of Nicotiana benthamiana and Verticillium dahliae interaction

Project description:Verticillium dahliae is a soil-borne fungus with a broad host range, including the model plants Nicotiana benthamiana and Arabidopsis thaliana. The plant immunity can be activated by Vd-derived patterns while V. dahliae secreted effectors. We report here the RNAseq analyses of samples from N. benthamiana infected by V. dahliae strains 592 and 171 at different time points. The data showed dynamic expression patterns of genes not only from plant defense signaling but also plant developmental signaling.

2023-07-13 | GSE236729 | GEO

Strigolactones negatively regulate Tobacco mosaic virus resistance in Nicotiana benthamiana

Project description:Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens, but there are currently no reports on their role in the interaction between Nicotiana benthamiana and Tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, an SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV-N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.

2024-08-16 | GSE272661 | GEO

Transcriptome of Zebrafish Intestine under Fe'i and Cavendish Banana Diet

Project description:Fe'i banana (Musa troglodytarum) fruit is carotenoid-rich and has been proposed to be utilized as a functional food. We report the first transcriptome of zebrafish (Danio rerio) intestine that were subjected to 8 weeks of Fe'i banana and Cavendish (Musa acuminata) diet. We found that Fe'i consumption in zebrafish modulated genes related to innate immunity.

2021-12-12 | GSE163139 | GEO

High throughput sequencing of the small RNAs from banana cv. Rasthali (AAB group)

Project description:Small RNAs comprise a group of non coding RNAs which play significant role in the genetic regulation of growth and development in plants. The most important members of this family are the small inhibiting RNAs (siRNAs) and microRNAs (miRNAs). They are processed by the DICER like proteins in plants and incorporated into the RNA induced silencing complex to silence the cognate mRNAs by sequence specific cleavage. High throughput sequencing strategies have enabled the identification of complete gamut of small RNA sequences present in a plant tissue at a particular point of time enabling the study of their biological significance in different plant tissues. The small RNA profile of each plant shows few plant conserved sequences, few species specific sequences and few novel RNA sequences characteristic of each cultivar. Here we have performed high throughput sequencing of small RNA population derived from leaves of an elite edible Indian cultivar of banana cv. Rasthali (‘Silk’ subgroup with AAB genotype) to identify the microRNAs and other important small RNAs being expressed in the leaves of banana cv Rasthali. Generation of complete small RNA profile of banana cv. Rasthali for comparison with similar databases

2015-02-07 | E-GEOD-65728 | biostudies-arrayexpress

RNA sequencing of NlCSP11-infiltrated Nicotiana benthamiana

Project description:In order to find the differentially expressed RNAs and signaling pathways related to the dwarf phenotype induced by NlCSP11, 35S-NlCSP11, 35S-XopQ and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed RNAs in the infiltrated leaves (NlCSP11_Z/XopQ_Z) and the upper uninfiltrated leaves (NlCSP11_U/XopQ_U) of N. benthamiana. KEGG annotation revealed a large number of differentially expressed RNAs involved in plant-pathogen interactions, indicating that NlCSP11 resulted in a more strong systemic resistance in N. benthamiana.

2024-05-30 | GSE267686 | GEO

Proteomics of different types of immunity in potato

Project description:Plants have a wide variety of ways to defend against pathogens. A commonly used model of the plant immune system divides it into a general response triggered by pathogen associated molecular patterns (PAMPs) and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI) and the second as effector-triggered immunity (ETI). We have performed a proteomical analysis of one PTI and two ETI models in potato and compared their effect on protein expression.

2019-10-17 | PXD012576 | Pride

RNA-Seq of Arabidopsis Col-0 and sid2 seedlings treated with L-Glu and urea

Project description:Plant resistance inducers (PRIs) are compounds that protect crops from diseases by activating plant immunity. The exogenous treatment with glutamate (Glu), an important amino acid for living organisms, was shown to induce resistance against fungal pathogen in rice and tomato. To understand the molecular mechanism of Glu-induced immunity, we developed a model system using Arabidopsis thaliana. Here, we found that exogenous treatment with Glu to Arabidopsis enhances resistance against Pseudomonas syringae pv. tomato DC3000 and Colletotrichum higginsianum. Consistently, transcriptome analyses of Arabidopsis seedlings treated with Glu showed that Glu significantly induces the expression of wound, defense, and stress related genes. Interestingly, Glu activates the expression of pathogen or damage associated molecular patterns (PAMP or DAMP)–inducible genes at much later time points than PAMP/DAMPs normally do. Moreover, expression of Glu-inducible genes does not require known components of PAMP receptor complex, glutamate receptors, salicylic acid-biosynthesis enzyme, or glutamate decarboxylase. In addition, Glu also enhances PAMP-inducible immune responses, such as production of reactive oxygen species and mitogen-activated protein kinase activation. These results show that Glu activates PAMP/DAMP-triggered immunity signaling pathway in a novel manner.

2019-12-06 | GSE123621 | GEO

RNA-Seq of Arabidopsis Col-0 seedlings treated with L-Glu, urea, SA, and distilled water.

2019-12-06 | GSE123620 | GEO

Transcriptome profiling of banana in response to growth-promoting rhizobacteria

Project description:We report the banana transcriptome profile in response to two distinct growth-promoting rhizobacteria, Bacillus amyloliquefaciens and Pseudomonas fluorescens. The goal of our study is to identify plant genes differentially regulated by rhizobacteria-plant interaction along time. At the same time, we show that despite these two rhizobacteria regulate distinct sets of genes, the same functional categories has been over-represented, such as transcription factor activity, response to stress and metabolic processes.

2016-04-21 | GSE80495 | GEO

Small RNA sequencing of NlCSP1-infiltrated Nicotiana benthamiana

Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.

2021-09-01 | GSE169282 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data