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Expression data from Pseudomonas putida KT2440 Tn5 mutants

ABSTRACT: We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30°C to 10°C. The 10°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044).

ORGANISM(S): Pseudomonas putida KT2440

PROVIDER: GSE28257 | GEO | 2014/02/01

SECONDARY ACCESSION(S): PRJNA139569

REPOSITORIES: GEO

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Expression data from Pseudomonas putida KT2440 Tn5 mutants

Project description:We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30Â°C to 10Â°C. The 10Â°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044). Cultures were grown in minimal medium supplemented with succinate at 30Â°C. In mid-exponential phase, cells were cooled down to 10Â°C. Two hours after the medium had reached 10Â°C, cells were harvested for subsequent RNA extraction.

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Project description:The metabolically versatile Pseudomonas putida strain KT2440 is the first Gram-negative soil bacterium certified as a biosafety strain and is being used for applications in agriculture, biotechnology and bioremediation. P. putida has to cope in its niche with numerous abiotic stresses. The stress response to 4°C, pH 4.5, 0.8 M urea or 45 mM sodium benzoate, respectively, was analyzed by the global mRNA expression profile and screening for stress-intolerant Tn5 transposon mutants. In total we identified 49 gene regions to be differentially expressed and 32 genes in 22 operons to be indispensable for growth during exposure to one or the other abiotic stresses. We propose that stress is sensed by the outer membrane proteins OmlA and FepA and the inner membrane constituents PtsP, PhoPQ and CbrAB. The metabolic response is regulated by the cyo operon, the RelA/SpoT modulon, PcnB and VacB that control mRNA stability and BipA that exerts transcript-specific translational control. The adaptation of the membrane barrier, the uptake of phosphate, the maintenance of intracellular pH and redox status and the translational control of metabolism are the indispensable key mechanisms of the P. putida stress response. Keywords: functional genomics

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