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Expression data from Pseudomonas putida KT2440 Tn5 mutants

ABSTRACT: We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30°C to 10°C. The 10°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044).

ORGANISM(S): Pseudomonas putida KT2440

PROVIDER: GSE28257 | GEO | 2014/02/01

SECONDARY ACCESSION(S): PRJNA139569

REPOSITORIES: GEO

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Expression data from Pseudomonas putida KT2440 Tn5 mutants

Project description:We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30Â°C to 10Â°C. The 10Â°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044). Cultures were grown in minimal medium supplemented with succinate at 30Â°C. In mid-exponential phase, cells were cooled down to 10Â°C. Two hours after the medium had reached 10Â°C, cells were harvested for subsequent RNA extraction.

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