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A single functional blast resistance gene Pi54 activates complex defense mechanism in Rice

ABSTRACT: To investigate the responses of resistance gene Pi54 at transcriptome level in blast resistant transgenic rice line TP-Pi54 during infection by Magnaporthe oryzae, we studied the co-expression analysis using microarray along with susceptible non-transgenic line Taipei 309 (TP). Suitable controls for both lines were used. The comparative analysis of differentially expressed genes was conducted.

ORGANISM(S): Oryza sativa Oryza sativa Japonica Group

PROVIDER: GSE28308 | GEO | 2011/12/08

SECONDARY ACCESSION(S): PRJNA139499

REPOSITORIES: GEO

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A single functional blast resistance gene Pi54 activates complex defense mechanism in Rice

Project description:To investigate the responses of resistance gene Pi54 at transcriptome level in blast resistant transgenic rice line TP-Pi54 during infection by Magnaporthe oryzae, we studied the co-expression analysis using microarray along with susceptible non-transgenic line Taipei 309 (TP). Suitable controls for both lines were used. The comparative analysis of differentially expressed genes was conducted. Total RNA from the leaves of mock inoculated non transgenic line Taipei 309 (TP) and transgenic line (TP-Pi54) along with challenge inoculated plants were isolated at 72 hours post inoculation. The samples were analyzed in two different biological replications (A and B) making total eight samples.

2011-12-08 | E-GEOD-28308 | biostudies-arrayexpress

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