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WT vs KO comX comparison

ABSTRACT: Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB003 (deletion of comX, STER_0189) for the identification of the ComX regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.4 for mRNA extraction

ORGANISM(S): Streptococcus thermophilus Streptococcus thermophilus LMD-9

PROVIDER: GSE28372 | GEO | 2012/01/31

SECONDARY ACCESSION(S): PRJNA139439

REPOSITORIES: GEO

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KO comR vs. KO comX comparison

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WT vs KO comX comparison

Project description:Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB003 (deletion of comX, STER_0189) for the identification of the ComX regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.4 for mRNA extraction wild-type vs knockout, 2 WT biological replicates (one used as technical replicate for a dye swap), 2 KO biological replicates (one used as technical replicate for a dye swap)

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Project description:Transcriptional profiling of S. thermophilus LMD-9 wild-type in the presence of mitomycin C. Cells were grown in CDM medium supplemented with lactose 1% (CDML), without or with mitomycin C 0.08 microg/ml, and sampled at OD600 = 0.4 for mRNA extraction. wild-type vs wild-type + mitomycin C, 2 WT biological replicates (one used as technical replicate for a dye swap), 2 WT + mitomycin C biological replicates (one used as technical replicate for a dye swap)

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mRNA transcriptional profiles in Streptococcus thermophilus wild-type strain LMD-9 and 3 mutants deleted for the pptAB, eep and amiCDE genes.

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