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Precise RNA targeting with CRISPR-Cas13d

ABSTRACT: The possibility of collateral RNA degradation poses a concern for transcriptome perturbations and therapeutic applications using CRISPR-Cas13. We show that collateral activity only occurs with high RfxCas13d expression. Using low-copy RfxCas13d in transcriptome-scale and combinatorial pooled screens, we achieve high on-target knockdown without extensive collateral activity. Further, analysis of a high-fidelity Cas13 variant suggests that its reduced collateral activity may be due to overall diminished nuclease capability.

ORGANISM(S): Homo sapiens

PROVIDER: GSE284017 | GEO | 2024/12/18

REPOSITORIES: GEO

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			Action	DRS
		Other
	GSE284017_DualTitration_counts.csv.gz	Csv
	GSE284017_Essentiality_HAP1_counts.csv.gz	Csv
	GSE284017_Essentiality_HEK_counts.csv.gz	Csv
	GSE284017_HFCas13d_counts.csv.gz	Csv

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