TIRE-seq: an Integrated Sample Extraction and Transcriptomics Workflow for High Throughput Perturbation Studies
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ABSTRACT: TIRE-seq (Turbocapture Integrated RNA Expression Sequencing) is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. Compared to traditional protocols like Primeseq, TIRE-seq demonstrates improved sequencing efficiency using crude cell lysates. We validated the method across three biological applications: analyzing transcriptional changes in stimulated human T cells, identifying genes driving murine dendritic cell differentiation, and examining temozolomide's dose-response effects on patient-derived neurospheres. TIRE-seq offers a streamlined, cost-effective approach for gene expression studies, reducing workflow complexity and minimizing sample loss.
ORGANISM(S): Mus musculus Homo sapiens
PROVIDER: GSE284395 | GEO | 2024/12/19
REPOSITORIES: GEO
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