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Transcriptome profile analysis in bovine blastocysts developed in vivo after intrafollicular transfer of matured or immature oocytes

ABSTRACT: This study aimed to investigate the transcriptome profile of bovine blastocysts produced in three different experimental conditions, namely I) VITRO (entire in vitro production of blastocysts), II) IFOT-M (Intrafollicular transfer of in vitro matured oocytes into preovulatory follicles) and III) IFOT-IM (Intrafollicular transfer of immature oocytes into dominant follicles) and ex vivo derived blastocysts (IV, VIVO) were used as a control. Accordingly, differentially expressed genes between in vitro and in vivo derived blastocysts which are involved in various fundamental roles including protein synthesis and protein degradation were identified. The study highlights insights about the attainment of embryonic developmental capacity and might be useful to improve embryo in vitro culture conditions.

ORGANISM(S): Bos taurus

PROVIDER: GSE284466 | GEO | 2024/12/30

REPOSITORIES: GEO

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Project description:Oocyte competence for early embryo development relies on intercellular communication between the maturing oocyte and preovulatory follicle. Preovulatory follicle maturity, as indicated by serum estradiol concentration or follicle diameter, has previously been linked to pregnancy, follicular fluid metabolites, cumulus-oocyte metabolism, and oocyte competency for embryo development. Such relationships indicate metabolic and developmental programming of the oocyte based on the preovulatory follicle’s physiological status, but downstream impacts on the molecular signature of blastocysts have not been examined. We hypothesized that supplementing maturing oocytes with follicular fluid originating from preovulatory follicles of greater or lesser maturity would impact the transcriptome of resulting blastocysts and indicate metabolic programming of the embryo that originated from the oocyte’s maturation environment. The objective was to investigate the effect of follicle maturity on metabolic preparation of the oocyte by examining the functional genome of blastocysts originating from oocytes matured in the presence of follicular fluid from preovulatory follicles of greater or lesser maturity. In vitro maturing oocytes were supplemented with follicular fluid collected from preovulatory follicles of greater or lesser maturity. Following identical embryo culture procedures, RNA-sequencing was performed on pools of 2 blastocysts (Greater, n = 12; Lesser, n = 15; all with stage code = 7 and quality code = 1). A total of 12,310 gene transcripts were identified in blastocysts after filtering to remove lowly abundant transcripts. There were 113 transcripts that differed in abundance between blastocysts originating from oocytes matured in greater versus lesser maturity follicular fluid (eFDR < 0.01). Although no pathways were significantly enriched with differentially abundant transcripts, transcriptome profiles suggested improved Wnt/β-catenin signaling, metabolism, and protection from oxidative stress in blastocysts derived from oocytes matured in greater maturity follicular fluid, while unregulated cell growth presented in blastocysts resulting from the lesser follicle maturity treatment.

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Transcriptome profile analysis in bovine blastocysts developed in vivo after intrafollicular transfer of matured or immature oocytes

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