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A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradients in vitro

ABSTRACT: Understanding how morphogen gradients spatially pattern tissues is a fundamental question in developmental biology but can be difficult to directly address using conventional approaches. Here we expose hPSC-derived endoderm cells to countervailing gradients of anteriorizing and posteriorizing signals using a widely available microfluidic device. This approach yielded spatially patterned cultures comprising anterior foregut (precursor to the thyroid, esophagus, and lungs) and mid/hindgut (precursor to the intestines) cells, whose identities were confirmed using single-cell RNA-sequencing. By exposing stem cells to externally applied signaling gradients, this widely accessible microfluidic platform should accelerate the production of spatially patterned tissues, complementing internally self-organizing organoids. Applying artificial morphogen gradients in vitro may also illuminate signaling gradient interpretation mechanisms that are otherwise challenging to study in mammalian embryos in vivo.

ORGANISM(S): Homo sapiens

PROVIDER: GSE285075 | GEO | 2024/12/25

REPOSITORIES: GEO

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A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradients in vitro

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