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Serum and Tear Autoantibodies from NOR Mice as Potential Diagnostic Indicators of Local and Systemic Inflammation in Sjögren's Disease [MCF_SH_292_Slide1_532]

ABSTRACT: Male Non-Obese Diabetic (NOD) derived NOR mice develop autoimmune dacryoadenitis by 16 weeks of age including lymphocytic infiltration of the lacrimal glands, reduced tear production, and increased cysteine tear proteases. Unlike the NOD mice, the NOR mice do not develop diabetes and studies designed with the NOR mice are expected to not have diabetes development counfounding the results observed for Sjogren's development. Autoantigens with high levels of reactivity in serum and/or tears of diseased mice may have diagnostic potential. Serum was collected from mice at 16 weeks old male NOR mice along with age matched male healthy BALBc controls. Approximately 0.8 mL of blood was collected by cardiac puncture into serum separator tubes. Following cold centrifugation, serum was collected. Tears were collected following topical stimulation of the lacrimal gland with carbachol. For tear samples, tears were pooled from 1, 2 or 3 mice, respectively to generate three samples. This was done mainly to optimize the number of mice required to obtain sufficient sample for the assay as well as to assess the sensitivity of mouse tears for the proteinarray. 1 to 10 uL of tear or 10 uL of serum per sample was used to determine reactivity against 94 autoantigens using proteinarray.

ORGANISM(S): blank sample Mus musculus

PROVIDER: GSE286502 | GEO | 2025/01/28

REPOSITORIES: GEO

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