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CO1 network analysis in poplar

ABSTRACT: We conducted microarray experiments by comparing constitutive constructs with appropriate controls, followed by the identification of downstream targets of Pro35S:CO1

ORGANISM(S): Populus sp. Populus tremula x Populus alba

PROVIDER: GSE28689 | GEO | 2011/12/29

SECONDARY ACCESSION(S): PRJNA143069

REPOSITORIES: GEO

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CO1 network analysis in poplar

Project description:We conducted microarray experiments by comparing constitutive constructs with appropriate controls, followed by the identification of downstream targets of Pro35S:CO1 Four samples of mature leaf tissues were collected from four independent lines of 35S:CO1 and pBI101. RNA was extracted from tissues and hybridized on Affymetrix Genechip Poplar Genome Array.

2011-12-29 | E-GEOD-28689 | biostudies-arrayexpress

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CO1 and CO2 network analysis in poplar

Project description:This SuperSeries is composed of the following subset Series: GSE28689: CO1 network analysis in poplar GSE28693: CO2 network analysis in poplar Refer to individual Series

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Overexpression of PtaHB1, Homeodomaine-leucine-Zipper class III, in hybrid poplar

Project description:Transcriptional profiling of primary xylem tranformed cells comparing control empty vector to PtaHB-1 overexpressed Over-expression constructs were obtained by inserting the complete coding sequences of PtaHB1 and PtaHB7 from P. tremula x P. alba between the maize ubiquitin promoter (Christensen et al., 1992) and a 35S terminator into the pCambia1305.2 vector (www.cambia.org). The resulting plasmids were then transferred into A. tumefaciens strain C58 pGV2260 (Hellens et al., 2000).

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Overexpression of PtaHB1, Homeodomaine-leucine-Zipper class III, in hybrid poplar

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Identification of Developing Xylem-Specific Promoters from Poplar by Using tissue type-Specific Whole Genome Transcriptome Analysis.

Project description:Lignocellulosic feedstock (i.e., wood) is gaining popularity as a source of fermentable sugars for liquid fuel production. To improve the quantity and quality of woody biomass, the developing xylem (DX) cell-specific genetic modification is desired. Bioinformatic analyses followed by the validation of cell type-specific transcriptomes led to the identification of 37 transcripts specifically expressed in DX. After further confirmation of DX-specific expression, we selected four genes (DX5, DX8, DX11 and DX15) to demonstrate the feasibility of our strategy. The promoter regions of selected DX genes were isolated and produced stable transformants of poplar by using transcriptional promoter:β-glucuronidase (GUS) fusion constructs. The GUS expression patterns of DX5 and ANAC073 (orthologous gene of Arabidopsis) revealed that these promoters were active in xylem cells in poplar at early seedling growth, and showed strongest expression in the developing xylem cells in the wood formation/development at later growth stages of poplar. DX specific and strong expression patterns of all the other DX promoters (DX8, DX11 and DX15) tested suggests that these promoters may be useful to control transgene expression in the DX cells of woody plants with the aim of the feedstock improvement.

2012-07-07 | GSE30507 | GEO

Identification of Developing Xylem-Specific Promoters from Poplar by Using tissue type-Specific Whole Genome Transcriptome Analysis.

Project description:Lignocellulosic feedstock (i.e., wood) is gaining popularity as a source of fermentable sugars for liquid fuel production. To improve the quantity and quality of woody biomass, the developing xylem (DX) cell-specific genetic modification is desired. Bioinformatic analyses followed by the validation of cell type-specific transcriptomes led to the identification of 37 transcripts specifically expressed in DX. After further confirmation of DX-specific expression, we selected four genes (DX5, DX8, DX11 and DX15) to demonstrate the feasibility of our strategy. The promoter regions of selected DX genes were isolated and produced stable transformants of poplar by using transcriptional promoter:?-glucuronidase (GUS) fusion constructs. The GUS expression patterns of DX5 and ANAC073 (orthologous gene of Arabidopsis) revealed that these promoters were active in xylem cells in poplar at early seedling growth, and showed strongest expression in the developing xylem cells in the wood formation/development at later growth stages of poplar. DX specific and strong expression patterns of all the other DX promoters (DX8, DX11 and DX15) tested suggests that these promoters may be useful to control transgene expression in the DX cells of woody plants with the aim of the feedstock improvement. In order to identify specifically and strongly expressed genes from DX cells of woody plant, we generated the tissue/cell type-specific transcriptomes from poplar stem by using whole genome GeneChip technology (Affymetrix).

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