Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity

ABSTRACT: Identification of IDH mutations has uncovered the crucial role played by metabolism in glioma-genesis. Oncolytic herpes virus (oHSV) therapy initiates direct tumor debulking by tumor lysis and also activates antitumor immunity however little is known about the role of glioma metabolism in determining oHSV efficacy. Here we identified that oHSV therapy rewired central carbon metabolism with increased glucose utilization towards oxidative phosphorylation and shuttled glutamine towards reductive carboxylation in IDH wildtype (wt) glioma. The switch in metabolism resulted in increased lipid synthesis, and cellular ROS. PKC induced ACSL4 in oHSV treated cells led to lipid peroxidation and ferroptosis. Ferroptosis was critical to launch an antitumor immune response important for efficacy. Mutant IDH (IDHR132H) gliomas are incapable of reductive carboxylation and hence ferroptosis. Pharmacological blockade of IDHR132H induced ferroptosis and antitumor immunity. This study provides a rationale to treat high grade IDHR132H glioma patients under oHSV treatment with IDHR132H inhibitor.

ORGANISM(S): Mus musculus

PROVIDER: GSE289317 | GEO | 2025/04/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity

Project description:IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity

| PRJNA1222406 | ENA

Single cell RNA-sequencing of M002 oHSV-treated murine glioblastoma

Project description:In this study, we evaluated the effects of M002 oncolytic herpes simplex virus (oHSV) in a syngeneic intracranial glioblastoma stem cell model. We harvested tumor-bearing mouse brain tissue 45 days post-injection of M002 oHSV and performed single-cell RNA-sequencing (scRNA-seq).

2024-12-03 | GSE246895 | GEO

oHSV Drives Tertiary Lymphoid Structure Boosting Oral Cancer Immunotherapy

Project description:The formation of TLSs induced by oncolytic virus treatment can enhance the anti-tumor response of oral cancer. Therefore, oncolytic virotherapy in vivo is a good strategy. To understand the mechanism of post-treatment changes, we collected oHSV-treated tumors for RNA-seq.

2025-01-08 | GSE262913 | GEO

Single cell RNA-sequencing of C134 oHSV-treated murine medulloblastoma

Project description:Here, we studied the effects of oHSV C134 in two syngeneic medulloblastoma mouse models, one that aligns with the sonic hedgehog (SHH) subtype (MYCN) and another that aligns with tumors of the group 3 subtype (CMYC).We harvested tumor tissue two and six days post-injection of C134 and performed single cell RNA-sequencing (scRNA-seq).

2023-07-27 | GSE200008 | GEO

Transcriptomic analysis of E0771 tumors treated with FEC + oHSV-1 Vs PBS

Project description:E0771 tumors were implanted either subcutaneously on the left flank or orthotopically in the mammary fat pad and treated with either PBS or FEC + oHSV-1 Mice were sacrificed 5 days after the start of treatment and whole tumour digests were used for RNA extraction

2021-10-01 | GSE183864 | GEO

Gene expression profiles of oHSV-infected U-251 MG cells in the presence or absence of LRRK2-IN-1

Project description:Gene expression profiles of oHSV-infected U-251 MG cells in the presence or absence of LRRK2-IN-1

2023-11-03 | GSE246606 | GEO

Reductive carboxylation epigenetically instructs effector T cell differentiation

Project description:Reductive carboxylation epigenetically instructs effector T cell differentiation

| PRJNA791337 | ENA

SIRT1-FOXO3 Pathway reprograms fatty acid metabolism to Mitigate Ferroptosis and Fibrosis [ChIP-seq]

Project description:Targeting ferroptosis with reductive agents has emerged as a promising strategy to mitigate fibrotic diseases in various pathological conditions. However, while the NAD+/NADH redox pair plays a crucial role in biological processes, its connection to ferroptosis beyond FSP1 remains poorly understood. To explore underappreciated NAD+/NADH-dependent factors in the ferroptosis cascade, we conducted a compound screening of 138 NAD+ enzyme modulatory molecules and identified Sirtuin-1 (SIRT1) activators as critical regulators of ferroptosis. SIRT1 activators, such as SRT1720, broadly inhibit ferroptosis by reducing lipid peroxidation without affecting labile iron levels. Moreover, SRT1720 effectively alleviates pulmonary and liver fibrosis in mouse models. Using ChIP-seq and validation assays, we demonstrated that FOXO3 binds to gene loci involved in fatty acid elongation and arachidonic acid metabolism in ferroptosis. SRT1720 disrupts this binding, thereby restraining fatty acid metabolism and limiting lipid peroxides production. Our findings establish the SIRT1-FOXO3 axis as a key inhibitory pathway against ferroptosis and highlight its therapeutic potential for ferroptosis-associated diseases, including fibrosis.

2025-03-26 | GSE292755 | GEO

SIRT1-FOXO3 Pathway reprograms fatty acid metabolism to Mitigate Ferroptosis and Fibrosis

2025-03-26 | GSE292618 | GEO

oHSV Drives Tertiary Lymphoid Structure Boosting Oral Cancer Immunotherapy

Project description:oHSV Drives Tertiary Lymphoid Structure Boosting Oral Cancer Immunotherapy

| PRJNA1094894 | ENA