Gene expression profiles in rat alveolar macrophages exposure to cellulose nanocrystals
Ontology highlight
ABSTRACT: To evaluate the biological impact of cellulose nanocrystals (CNCs) at the transcriptional level, we conducted whole-genome microarray analyses on rat alveolar macrophages (NR8383) exposed to CNCs with different physicochemical properties.
Project description:To assess the biological impact of cellulose nanofibrils (CNFs) at the transcriptional level, we conducted whole-genome microarray analyses on rat alveolar macrophages (NR8383) exposed to CNFs with varying physicochemical properties. The findings were compared with those from exposure to microcrystalline cellulose (MCC) and lipopolysaccharide (LPS).
Project description:To evaluate the biological effects at the transcriptional level of manufactured nanomaterials, we performed whole genome microarrays of rat alveolar macrophages (NR8383) exposed to multi-walled carbon nanotubes (MWCNTs) with different physicochemical properties.
Project description:To evaluate the biological effects at the transcriptional level of manufactured nanomaterials, whole genome microarrays of rat alveolar macrophages (NR8383) exposed to three types of submicron-diameter carbon fibers (SCFs) with different physicochemical properties were performed. In comparison with the fibers of SCF1, the fibers of SCF2 were shorter, and the fibers of SCF3 were thinner.
Project description:Gene expression profiles in rat alveolar macrophages exposure to cellulose nanofibrils, microcrystalline cellulose, and lipopolysaccharide.
Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of single-wall carbon nanotubes (SWCNTs). Two kinds of the impurity-free singlewall carbon nanotubes (SWCNTs), CNT-1 or CNT-2 induced gene expression in rat alveolar macrophages were measured at 24 hours exposure to doses of 0.1mg/mL. The experiments were performed using control for each experiment (n=4).
Project description:Gene expression profiling of the rat alveolar macrophages (NR8383) and human alveolar epithelial cell line (A549) exposure to iron-enriched pristine single-wall carbon nanotubes (SWCNTs) or their purified SWCNTs were employed to gain insights at the transcriptional level.
Project description:To evaluate the biological effects at the transcriptional level of manufactured nanomaterials, whole genome microarray analysis of rat lungs intratracheally instilled with cellulose nanofibrils (CNFs) with different physicochemical properties or multi-walled carbon nanotubes (MWCNTs) were performed.
Project description:To evaluate the biological impact of cellulose nanofibrils (CNFs) at the transcriptional level, we conducted whole-genome microarray analyses on human bronchial epithelial cells (BEAS-2B) exposed to CNFs with different physicochemical properties. The results were compared with those from exposures to microcrystalline cellulose (MCC).
Project description:In this study, alveolar macrophages (cell line NR8383) were treated with three different doses of nanomaterials (SiO2_15_Amino, SiO2_15_Unmod, SiO2_7, SiO2_40, TiO2_NM105) for 24 h. The effects were investigated using untargeted proteomics.
Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of exfoliated graphene (EGr).